Sequences from confirmation amplicon


Confirmation sequences for line 718D10 and hit At2g41960 are shown.
Line ID 718D10
Locus At2g41960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17514609
Primers used for PCR 16ie - 8474, show primer details
   
Sequence Name 43-K112758-0022-718-D10C-16ie
GenBank Accession LN504894 [GenBank]
Sequencing primer gene specific primer, 16ie: 5'-AGTCTCTTGGTCTTCAGCTTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112758-0022-718-D10C-16ie
ATACACTGGTCCCCTTGGATACAGTCTGAATGGAGAGTCTCCTTTATGCATGCAGTACGG
GACCCCATTGAACCATTCAGCAGCTCCTTTCTTCAATTCCGGGCCAGTTCCAATTTTCCA
TCCGTTTGCTGAAACCAACACAAAAAGATCAGCAAAATCGAAAGGATGTCCATTGTAAAT
GATCAAGAGTTACTGTTGCTGTAGATCAACAACAATAGCTCATACAATCGAAAAAGAAAA
ATGTAACCTCCAGGGACATGTGTATACACATGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTATT
   
Sequence Name 03-K112757-0022-718-D10C-8409
GenBank Accession LN487558 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112757-0022-718-D10C-8409
CATTTCATTGAAATATCCATGTGTATACACATGTCCCTGGAGGTTACATTTTTCTTTTTC
GATTGTATGAGCTATTGTTGTTGATCTACAGCAACAGTAACTCTTGATCATTTACAATGG
ACATCCTTTCGATTTTGCTGATCTTTTTGTGTTGGTTTCAGCAAACGGATGGAAAATTGG
AACTGGCCCGGAATTGAAGAAAGGAGCTGCTGAATGGTTCAATGGGGTCCCGTACTGCAT
GCATAAAGGAGACTCTCCATTCAGACTGTATCCAAGGGGACCAGTGTATACATAGTGGTT
AGGATGAGGGAATTGCATTAATCCGTTGGTTGAAGCTGAAGACCAAGAGAC

Last Updated on 06.12.2018 16:11