Sequences from confirmation amplicon


Confirmation sequences for line 749A05 and hit At2g43460 are shown.
Line ID 749A05
Locus At2g43460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18046818
Primers used for PCR 39wc - 8474, show primer details
   
Sequence Name 71-K118585-0022-749-A05C-39wc
GenBank Accession LN512634 [GenBank]
Sequencing primer gene specific primer, 39wc: 5'-ATTTCAGGTTTACGAATTAGGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118585-0022-749-A05C-39wc
CCTCGCGACGGCGAATTCGCTCGACTCTCTCTGTGGACGGCGGCTAGTTTCAACCAACAG
ATTCGAGAATGGTACAATATTTCTCGGTCTTTCGATTTCGTTTCATATCGCCGTCATGGA
TGCTTATGATTTGGTTTTCATAGCTCGTTTGCAATCATTCATAGTGTAGTAGAGCTGTTC
CTGTATCTTAAGAGTTTTCTCAAAAAATGTAGATTTTTGTATCATTTTCAGCTTTGATTA
TTGCTTCTTACTTTGTTTAGGATATAGTGAAACGTGTAGGTTTTAGTGAATTAACCAAAA
ACCTAGGTCAGAGTTTGCTTAATGTGTTTGATGTGCTGAGCTCGTGTCTGCTTATCATGT
GATTAACTTTTGATTGGTTGGGATTATTGAATCTTGTATTGAATTTTGATTGATTAATTT
GATTAATGTTTGCAGCCTAAGCAAATCCACGAGATCAAGGACTTCCTTCTGACAGCTAGA
AGGAAAGATGCACGATCTGTGAAGATCAAGAGAAGCAAGGATATTGTCAAGTTTAAGGTC
AGACAAGAAATCCGACCAATTACATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 41-K118584-0022-749-A05C-8409
GenBank Accession LN504263 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118584-0022-749-A05C-8409
TGTATTGGTCGGATTTCTTGTCTGACCTTAACTTGACAATATCCTTGCTTCTCTTGATCT
TCACAGATCGTGCATCTTTCCTTCTAGCTGTCAGAAGGAAGTCCTTGATCTCGTGGATTT
GCTTAGGCTGCAAACATTAATCAAATTAATCAATCAAAATTCAATACAAGATTCAATAAT
CCCAACCAATCAAAAGTTAATCACATGATAAGCAGACACGAGCTCAGCACATCAAACACA
TTAAGCAAACTCTGACCTAGGTTTTTGGTTAATTCACTAAAACCTACACGTTTCACTATA
TCCTAAACAAAGTAAGAAGCAATAATCAAAGCTGAAAATGATACAAAAATCTACATTTTT
TGAGAAAACTCTTAAGATACAGGAACAGCTCTACTACACTATGAATGATTGCAAACGAGC
TATGAAAACCAAATCATAAGCATCCATGACGGCGATATGAAACGAAATCGAAAGACCGAG
AAATATTGTACCATTCTCGAATCTGTTGGTTGAAACTAGCCGCCGTCCACAGAGAGAGTC
GAGCGAATTCGCCGTCGCGAGGATATAAAGAGATCTCTTAAAAACTTTGTATCAAGAAGC
CCTAATTCGTAACCCCTGAAA

Last Updated on 06.12.2018 16:11