Sequences from confirmation amplicon


Confirmation sequences for line 828H05 and hit At2g44050 are shown.
Line ID 828H05
Locus At2g44050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18224283
Primers used for PCR BC29 - 8474, show primer details
   
Sequence Name 44-K103981-0022-828-H05C-BC29
GenBank Accession LN505163 [GenBank]
Sequencing primer gene specific primer, BC29: 5'-CAGGAACCCAAATAACCTGAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103981-0022-828-H05C-BC29
GAAGCTCAAACTTTTTATTCTGTTCTACAAGCAAAAGACTTTTACTTCACTGTATTCTTC
TCTGACTGAATACTTCTTGAAAGTCTCAATCGCTCCTTCCAAAAGCAACTTAGTCACAAC
CTCATTGAAACGAGCTACCACCTTGAGAAAGAAAAATGAATCAGAACCAAGTCAACACTG
CAATTGAGCTCAAAGTTTTCAAATTTCACTGTAAATCAGAACCAAGTCACTAAATCTAAG
CAGCGCACAAAGTAAGAAAGCGATTCTGAGAACTCACGATGGCGAATCTAAGACCTTCGC
CTCTGATAAGAGACCCCGTAACATGGCGAACAGCAGCCGTCTGTACGAATGAAGAGCGTA
ATTCCTTCTCTACAGCTAGTGGTGACGCAAATCCTAACACCAAATGTTAACATCTCAATC
ACGAAATTGCGAAAAAACGATTTGAGTTATTATAAGAGAAGTGAGAGAAAGCAAGCTTAC
CGGATGTGGATGAGGAGAATGAGAGATTATTGGATTTGTTCACAGAAGAGCCACCGTGTA
TATAACAGGCGGAGGAAGATTGACGCGAATTGAGCTGACGGTGTGCCGTCGGTATCAGGC
GGAGACACGGCGGCGAAACTAATGACTTCATTTTTCGTCGTCGGAGTT
   
Sequence Name 60-K103982-0022-828-H05C-8409
GenBank Accession LN510244 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103982-0022-828-H05C-8409
CCATTTAAATTGAAATATCCTGTAAAACTCCGACGAACGACCATATAAGTCATTAGCTTC
GCCGCCGTGTCTCCGCCTGATACCTACGGCACACCGTCAGCTCAATTCGCGTCAATCTTC
CTCCGCCTGTTATATACACGGTGGCTCTTCTGTGAACAAATCCAATAATCTCTCATTCTC
CTCATCCACATCCGGTAAGCTTGCTTTCTCTCACTTCTCTTATAATAACTCAAATCGTTT
TTTCGCAATTTCGTGATTGAGATGTTAACATTTGGTGTTAGGATTTGCGTCACCACTAGC
TGTAGAGAAGGAATTACGCTCTTCATTCGTACAGACGGCTGCTGTTCGCCATGTTACGGG
GTCTCTTATCAGAGGCGAAGGTCTTAGATTCGCCATCGTGAGTTCTCAGAATCGCTTTCT
TACTTTGTGCGCTGCTTAGATTTAGTGACTTGGTTCTGATTTACAGTGAAATTTGAAAAC
TTTGAGCTCAATTGCAGTGTTGACTTGGTTCTGATTCATTTTTCTTTCTCAAGGTGGTAG
CTCGTTTCAATGAGGTTGTGACTAAGTTGCTTTTGGAAGGAGCGATTGAGACTTTCAG

Last Updated on 06.12.2018 16:11