Sequences from confirmation amplicon


Confirmation sequences for line 939F02 and hit At2g44540 are shown.
Line ID 939F02
Locus At2g44540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18387585
Primers used for PCR 39xw - 3144, show primer details
   
Sequence Name 34-K119512-0022-939-F02C-39xw
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 39xw: 5'-TTTCCATTGCTCCCTACTTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119512-0022-939-F02C-39xw
GTTTGTTTCTTGCCACATAAGATAGTGATATGTTTTCTTTAGTGAAATAGTCATAACTTA
CCTTGGCCATCAAGACTTGTGCACCCACGAACTTATCGTCCCAGGCAAAAACACTTCTTG
GACCTCCGGTACCAGATGCTTGTGTTAGGTAATTGAGATAAATCTGGTCGTTAGTAGCAC
GGTGGAGCCAAGCTGCAGCCCACAACAACTCATCTTCGTATCCACTACTCGCGTAGAAGC
CACCCGCGTTAGTAATGGAGTTTTGGTAAAGGCCAGGGTGAGCCTTAGCGAATGCAAATA
AATCTTTTGCATGACCAATGAGTATATTGGCATAGGCTTTATCAAATGGTGCGAAGGCGA
TAGACGCTGCCGCCATTGCGGCAGCTGTCTCACCTGCTAGGTCAGAACCTGGATGTTGAG
CATCGATGCGGTATGAAGTACGCGGAGTGGTCATATCCTCAGGTCTCATCCAGCATTTGT
GGTCCGAGTATGATGGTCCGAGTATGATGGTCATCAAACACTGATAGTTTAAACCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCACATCAATCCAC
   
Sequence Name 02-K119511-0022-939-F02C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K119511-0022-939-F02C-3144
TCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATGACCATCA
TACTCGGACCATCATACTCGGACCACAAATGCTGGATGAGACCTGAGGATATGACCACTC
CGCGTACTTCATACCGCATCGATGCTCAACATCCAGGTTCTGACCTAGCAGGTGAGACAG
CTGCCGCAATGGCGGCAGCGTCTATCGCCTTCGCACCATTTGATAAAGCCTATGCCAATA
TACTCATTGGTCATGCAAAAGATTTATTTGCATTCGCTAAGGCTCACCCTGGCCTTTACC
AAAACTCCATTACTAACGCGGGTGGCTTCTACGCGAGTAGTGGATACGAAGATGAGTTGT
TGTGGGCTGCAGCTTGGCTCCACCGTGCTACTAACGACCAGATTTATCTCAATTACCTAA
CACAAGCATCTGGTACCGGAGGTCCAAGAAGTGTTTTTGCCTGGGACGATAAGTTCGTGG
GTGCACAAGTCTTGATGGCCAAGGTAAGTTATGACTATTTCACTAAAGAAAACATATCAC
TATCTTATGTGGCAAGAAACAAACATAACACAAAGTTTTGCAAATGCAGCTTGCACTTGA
ACGGANAGTAG
Primers used for PCR 51xw - 8474, show primer details
   
Sequence Name 35-K119512-0022-939-F02C-51xw
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 51xw: 5'-GTGAATACATACATAGACATAGACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K119512-0022-939-F02C-51xw
GTAAATCTATTGTACAATTGGTCTTGAGATCGTCTAAAGATTTATCAAAAAAAGATGTCT
CAATCGAAAAACGGATCTTCACAATGCTTATGGACAAGTATTTGCATTGTGGTACTAGTA
ATGTCGATGGCTCGTGGAGCTGTTTCTACAAATTATGCAGAAGCTCTCCAAAAGAGTCTT
TTGTATTTTGAAGCTCAACGTTCTGGAAAATTGCCTCCGAACCAAAAAGTTACATGGAGA
GGAGATTCTGCACTCAAAGATGGTTCTGATGCTCATGTAAGTTTAATATCCAATTACTTA
TTTTTATTCCATATTTTTTTGTGTAATGAATATTTATTACTTGAAGTAAACTGTTTGCTA
ATTGTAAAAATAGATTGATCTCACCGGAGGGTATTACGATGCCGGAGACAATATGAAGTT
TGGATTTCCTTTAGCGTTCACTACGACGATGCTAGCTTGGAGCAACATAGAGATGGGATC
AGAGCTTAGGGCCCACCACGAGCAAGGAAACTCGCTCAGGGCTCTCAAGTGGGCCACCGA
TTATCTCATTAAAGCCCATCCTCAGCCTAATGTCCTGTACGGACAAGTGGCTTCGTGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGC
   
Sequence Name 03-K119511-0022-939-F02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119511-0022-939-F02C-8409
GTACAGGACATTAGGCTGAGGATGGGCTTTAATGAGATAATCGGTGGCCCACTTGAGAGC
CCTGAGCGAGTTTCCTTGCTCGTGGTGGGCCCTAAGCTCTGATCCCATCTCTATGTTGCT
CCAAGCTAGCATCGTCGTAGTGAACGCTAAAGGAAATCCAAACTTCATATTGTCTCCGGC
ATCGTAATACCCTCCGGTGAGATCAATCTATTTTTACAATTAGCAAACAGTTTACTTCAA
GTAATAAATATTCATTACACAAAAAAATATGGAATAAAAATAAGTAATTGGATATTAAAC
TTACATGAGCATCAGAACCATCTTTGAGTGCAGAATCTCCTCTCCATGTAACTTTTTGGT
TCGGAGGCAATTTTCCAGAACGTTGAGCTTCAAAATACAAAAGACTCTTTTGGAGAGCTT
CTGCATAATTTGTAGAAACAGCTCCACGAGCCATCGACATTACTAGTACCACAATGCAAA
TACTTGTCCATAAGCATTGTGAAGATCCGTTTTTCGATTGAGACATCTTTTTTTGATAAA
TCTTTAGACGATCTCAAGACCAATTGTACAATAGATTTTACTTTTTGCTTGTGTTTTGGA
TGTCTATGTCTATGTGGGTATTCA

Last Updated on 06.12.2018 16:11