Sequences from confirmation amplicon


Confirmation sequences for line 671B01 and hit At2g44730 are shown.
Line ID 671B01
Locus At2g44730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18437455
Primers used for PCR MS81 - 8474, show primer details
   
Sequence Name 02-K027155-022-671-B01C-8409
GenBank Accession LN486890 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K027155-022-671-B01C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTGGTAATTACTCTTTCTTCTT
CTTGTCTTGCTCCTCCGTAGTAGTATAATCTGATAAGCTTTTAGCAAACGCTTCCACAAT
TCGTTGTTGAGATTCCAAAATCATCTTTGTCCTCTTCATCTCTGTATCCATCCTCATCGC
TTCTATCTCTCTCGTCATCTCCATTCTCGTCTGCTCTGTTCTCACCAACGTATCTCCAAG
CAGCTTTATCGCACTAGCTATCTCCACCATTGGATCATTATCATCCTCCTCCGTCTTCAT
CATCATCTCTCTCCCTCGTTTCCCTCCTCCTTCAGGAATTCTCACAACTCTAGCACCGTA
ACTAGATCCTCCTGCTCCGATACCACGACCAGCTCTCGGATTTGAACTCACACCAGCACT
TGGACTCGCCGTGTATTTCTGATCAATCTTACCGGGAAACCTCGGTCCAGGCTGTGCTAT
ACTCACTCCAGTCGGTATCCGAATTCGAATCCCGCCGCTACTACTACTCCCTCCACCACC
ACCAGATCCAGCTGTACCATTCCTGTTAAAGAACCTCGGCGTCGTCCTCGCTCCTCCTCC
TCCGCCGCTGAATTGATACCTAGCTGTGTAATTACCATCATCGTGATCATCATCATCTCC
ACTTTCGTTACCTTGTTTAATCTCTGGTCTATTCTCCATAGCTTCC
   
Sequence Name 06-K027155-022-671-B01C-MS81
GenBank Accession LN488660 [GenBank]
Sequencing primer gene specific primer, MS81: 5'-TCATTTTAAGCGAATGGAAGCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K027155-022-671-B01C-MS81
AATAACCAGAGATTAAACAAGGTAACGAAAGTGGAGATGATGATGATCACGATGATGGTA
ATTACACAGCTAGGTATCAATTCAGCGGCGGAGGAGGAGGAGCGAGGACGACGCCGAGGT
TCTTTAACAGGAATGGTACAGCTGGATCTGGTGGTGGTGGAGGGAGTAGTAGTAGCGGCG
GGATTCGAATTCGGATACCGACTGGAGTGAGTATAGCACAGCCTGGACCGAGGTTTCCCG
GTAAGATTGATCAGAAATACACGGCGAGTCCAAGTGCTGGTGTGAGTTCAAATCCGAGAG
CTGGTCGTGGTATCGGAGCAGGAGGATCTAGTTACGGTGCTAGAGTTGTGAGAATTCCTG
AAGGAGGAGGGAAACGAGGGAGAGAGATGATGATGAAGACGGAGGAGGATGATAATGATC
CAATGGTGGAGATAGCTAGTGCGATAAAGCTGCTTGGAGATACGTTGGTGAGAACAGAGC
AGACGAGAATGGAGATGACGAGAGAGATAGAAGCGATGAGGATGGATACAGAGATGAAGA
GGACAAAGATGATTTTGGAATCTCAACAACGAATTGTGGAAGCGTTTGCTAAAAGCTTAT
CAGATTATACTACTACGGAGGAGCAAGACAAGAAGAAGAAAGAAGTGATTCCAATGGCTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11