Sequences from confirmation amplicon


Confirmation sequences for line 719C03 and hit At2g45970 are shown.
Line ID 719C03
Locus At2g45970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18913323
Primers used for PCR MG26 - 8474, show primer details
   
Sequence Name 16-K031455-022-719-C03C-MG26
GenBank Accession LN493320 [GenBank]
Sequencing primer gene specific primer, MG26: 5'-TGATTTCATTTGTAGATACGCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K031455-022-719-C03C-MG26
GGCAGCATTGACGCAACGAATTAAACGGATCATGATTGATGAATCTCCCACCGTCCGATT
GCGAAATCCATCTCTCTGGTTTGAATTCCAAACAATCCTCTCCCCAAGTCGATTTCATCC
TCCCCGCAGAGTAAATCGAATAAGTAATCGAAGATCCCGCCGGCACAAATGTCCCGTCCG
GTAACACATCGTCTTTCACGGCGCGTTTTGAATCCTCCGGCACCGAAGGATAAAGCCTCA
AAGTCTCCGATAACGCTGCCTTGAGAAAAACCAATCGATCAAGCTCTTCGCACGAAAGCG
GCTCGTCCGTCCATAACGCCACATCATCGCCACGTGTCTCAACCAGAACGGTACAGATCT
CACGGAGGATTTTATCCTCTATGGCCGGATGCTGCGTAATCAACCAGAAGAACCAGCTCA
GCGCGACTGATGACGTGTCACGTCCAGCTAGGATGAAGTTGAGCGCCACGCGCTGGAGGG
TCTCGTCGGAGTAAGACTCCTTCTTCTTGATGAACCGCGATACACACGTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACATTC
   
Sequence Name 08-K031455-022-719-C03C-8409
GenBank Accession LN489616 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K031455-022-719-C03C-8409
CCATTTACCGTGTGTATCGCGGTTCATCAAGAAGAAGGAGTCTTACTCCGACGAGACCCT
CCAGCGCGTGGCGCTCAACTTCATCCTAGCTGGACGTGACACGTCATCAGTCGCGCTGAG
CTGGTTCTTCTGGTTGATTACGCAGCATCCGGCCATAGAGGATAAAATCCTCCGTGAGAT
CTGTACCGTTCTGGTTGAGACACGTGGCGATGATGTGGCGTTATGGACGGACGAGCCGCT
TTCGTGCGAAGAGCTTGATCGATTGGTTTTTCTCAAGGCAGCGTTATCGGAGACTTTGAG
GCTTTATCCTTCGGTGCCGGAGGATTCAAAACGCGCCGTGAAAGACGATGTGTTACCGGA
CGGGACATTTGTGCCGGCGGGATCTTCGATTACTTATTCGATTTACTCTGCGGGGAGGAT
GAAATCGACTTGGGGAGAGGATTGTTTGGAATTCAAACCAGAGAGATGGATTTCGCAATC
GGACGGTGGGAGATTCATCAATCATGATCCGTTTAAATTCGTTGCGTTCAATGCTGGCCC
TAGGATCTGTCTGGGTAAGGATCTGGCGTATCTACAAATGAAATC

Last Updated on 06.12.2018 16:11