Sequences from confirmation amplicon


Confirmation sequences for line 366C01 and hit At2g46455 are shown.
Line ID 366C01
Locus At2g46455 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19071123
Primers used for PCR 14pq - 8474, show primer details
   
Sequence Name 10-K115927-0022-366-C01C-14pq
GenBank Accession LN490806 [GenBank]
Sequencing primer gene specific primer, 14pq: 5'-CCCCACTAATTGAACAAAAGACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115927-0022-366-C01C-14pq
GCTACGAATCAGAAGACTTGAATCTGGGAAAGTGGGTTCAGTAAAATCTTTGATATGATG
ATGAAGAACAACAGGTCTTGAGGTCATGTAGCATAACAAATTTGGGTAAAGACTTCTCCT
TGATAAGCTACGAAGACAGGCCATTTCTAGAGGTGAGCAAAGAAGTAACGCTGAGCTAAA
AAAGAAGAAAGATTGCAACTTGGGATCGCGAGTGGGTGATTTCTTACACGAATCTAGACA
AAGAGACACATCTTAACCAGAGATTTTCCCCAAATTTCAAAATGAAACAGAAACCCTAAG
TTTCCACAGGATAAAGAGATACACAGTGGTTCCCCCATTTGAAGCAGAAAAAGCACGCAA
AAGTAATGATTTTTCACAATTTCGTAACTCTTCGTACTCTGCTTTCCGCCATTTTTCTGC
ACTAATTTCCACTTTTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCG
   
Sequence Name 02-K115926-0022-366-C01C-8409
GenBank Accession LN487178 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115926-0022-366-C01C-8409
AAGTGGAATTAGTGCAGAAAAATGGCGGAAAGCAGAGTACGAAGAGTTACGAAATTGTGA
AAAATCATTACTTTTGCGTGCTTTTTCTGCTTCAATGGGGGAACCACTGTGTATCTCTTT
ATCCTGTGGAAACTTAGGGTTTCTGTTTCATTTTGAAATTTGGGGAAAATCTCTGGTTAA
GATGTGTCTCTTTGTCTAGATTCGTGTAAGAAATCACCCACTCGCGATCCCAAGTTGCAA
TCTTTCTTCTTTTTTAGCTCAGCGTTACTTCTTTGCTCACCTCTAGAAATGGCCTGTCTT
CGTAGCTTATCAAGGAGAAGTCTTTACCCAAATTTGTTATGCTACATGACCTCAAGACCT
GTTGTTCTTCATCATCATATCAAAGATTTTACTGAACCCACTTTCCCAGATTCAAGTCTT
CTGATTCGTAGCTTTCACAGGCATGTTTTCTTTGAAGATACAAGGAAAATGTCTTTTGTT
CATTTAGTGGGG

Last Updated on 06.12.2018 16:11