Sequences from confirmation amplicon


Confirmation sequences for line 283A09 and hit At2g47240 are shown.
Line ID 283A09
Locus At2g47240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19395733
Primers used for PCR 79cs - 8474, show primer details
   
Sequence Name 53-K108895-0022-283-A09C-79cs
GenBank Accession LN508181 [GenBank]
Sequencing primer gene specific primer, 79cs: 5'-ATATAAAATGACAGGCAACCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K108895-0022-283-A09C-79cs
GTGAGATTAAATGAATTAGGTGCACACACAAAGCGACGTTGATATTACCATAAATATCTT
TTTGTTGACTATTTGATCGTTGACCAGGGACGTGAGAAACCGGAAGACACGAACCCGCCT
AAGGCGTTTAACATATGCACCATAATGTACACCAGCGGCACAAGCGGTGATCCTAAAGGT
GTGGTTTTGACTCACCAAGCGGTCGCGACTTTTGTTGTTGGGATGGATCTCTATATGGAC
CAGTTTGAAGATAAGGTAACCCATAATTACTTCAAAGGAACAGTAGACACTTTTTAACCA
AAATTCAAATAAATTCTGAGACATAGAAAAATTTAGATATTTTGACATTTATAATGATAA
CTACTAATAAAATCGTAGAGAACAAATATTATCTGATTACTATTGATTAATCCTCTGTTT
ACTTATGACTAATCCTCTGTTTTCTTTGGGCGTCGGCCAGGAAATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 37-K108894-0022-283-A09C-8409
GenBank Accession LN502644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K108894-0022-283-A09C-8409
GCCATTTACAATTGAATATTTCCTGGCCGACGCCCAAAGAAAACAGAGGATTAGTCATAA
GTAAACAGAGGATTAATCAATAGTAATCAGATAATATTTGTTCTCTACGATTTTATTAGT
AGTTATCATTATAAATGTCAAAATATCTAAATTTTTCTATGTCTCAGAATTTATTTGAAT
TTTGGTTAAAAAGTGTCTACTGTTCCTTTGAAGTAATTATGGGTTACCTTATCTTCAAAC
TGGTCCATATAGAGATCCATCCCAACAACAAAAGTCGCGACCGCTTGGTGAGTCAAAACC
ACACCTTTAGGATCACCGCTTGTGCCGCTGGTGTACATTATGGTGCATATGTTAAACGCC
TTAGGCGGGTTCGTGTCTTCCGGTTTCTCACGTCCCTGGTCAACGATCAAATAGTCAACA
AAAAGATATTTATGGTAATATCAACGTCGCTTTGTGTGTGCACCTAATTCATTTAATTCT
CACATACACTCTAGTACCTAATGCCGTCAAGTTGGGTTGCCTGTCATTTTATA

Last Updated on 06.12.2018 16:11