Sequences from confirmation amplicon


Confirmation sequences for line 179G06 and hit At2g47630 are shown.
Line ID 179G06
Locus At2g47630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19536930
Primers used for PCR 14nb - 8474, show primer details
   
Sequence Name 10-K114418-0022-179-G06C-8409
GenBank Accession LN490761 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114418-0022-179-G06C-8409
CATTTCATTGAATATATCCATTGCCTTGATAAAATTTTATTACACAGTGGATCGTGGAAC
CAAACGGCCACGGTTCAAACCACAAAGGAGATTGAAGAAGGGACGTTTAGGCCTCCTATG
ACCGTTACTAACGCCGTTAACAGTAGTTACCCTCTGGACGCTCGTAGTGTTACCAACTCG
TATAGGAGTGACAGTGAGAGAGGCAGGGTCAGCGGTTCGAAGGTCAAGCCAATTGATGAT
GTCAGCAAAAACAAGGTCCACGTTACAGTCAGGCTCACCTGACGTCAGAGCGTGCCACAT
CCCTGGATACAACTTGAGCGTCTTGTCCCTTGTGCTAGCTTTCTCGTACAGAGCTTTGCT
TACCTCTGGATCCGTCACTGTATCTGCTTCTCCGTGTAATACAAAGAATGGCATTGTTAT
CTGCAATATCATGTTGTCTTGGTCAGAAATAATGTCATAACACGGGATCAAGAACAATCA
ATAAGAGACATGAACCTCGT
   
Sequence Name 34-K114419-0022-179-G06C-14nb
GenBank Accession LN501507 [GenBank]
Sequencing primer gene specific primer, 14nb: 5'-CACGAGGTTCATGTCTCTTATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K114419-0022-179-G06C-14nb
GACACATGATATTGCAGATAACATGCCATTCTTTGTATTACACGGAGAAGCAGATACAGT
GACGGATCCAGAGGTAAGCAAAGCTCTGTACGAGAAAGCTAGCACAAGGGACAAGACGCT
CAAGTTGTATCCAGGGATGTGGCACGCTCTGACGTCAGGTGAGCCTGACTGTAACGTGGA
CCTTGTTTTTGCTGACATCATCAATTGGCTTGACCTTCGAACCGCTGACCCTGCCTCTCT
CACTGTCACTCCTATACGAGTTGGTAACACTACGAGCGTCCAGAGGGTAACTACTGTTAA
CGGCGTTAGTAACGGTCATAGGAGGCCTAAACGTCCCTTCTTCAATCTCCTTTGTGGTTT
GAACCGTGGCCGTTTGGTTCCACGATCCACTGTGTAATAAAATTTTATCAAGGCAATGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCC

Last Updated on 06.12.2018 16:11