Sequences from confirmation amplicon


Confirmation sequences for line 301C06 and hit At2g47630 are shown.
Line ID 301C06
Locus At2g47630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19536999
Primers used for PCR 14nb - 8474, show primer details
   
Sequence Name 32-K115371-0022-301-C06C-14nb
GenBank Accession LN500706 [GenBank]
Sequencing primer gene specific primer, 14nb: 5'-CACGAGGTTCATGTCTCTTATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115371-0022-301-C06C-14nb
GACAACATGATATTGCAGATAACATGCCATTCTTTGTATTACACGGAGAAGCAGATACAG
TGACGGATCCAGAGGTAAGCAAAGCTCTGTACGAGAAAGCTAGCACAAGGGACAAGACGC
TCAAGTTGTATCCAGGGATGTGGCACGCTCTGACGTCAGGTGAGCCTGACTGTAACGTGG
ACCTTGTTTTTGCTGACATCATCAATTGGCTTGACCTTCGAACCGCTGACCCTGCCTCTC
TCACTGTCACTCCTATACGAGTTGGTAACACTACGAGCGTCCAGAGGGTAACTACTGTTA
ACGGCGTTAGTAACGGTCATAGGAGGCCTAAACGTCCCTTCTTCAATCTCCTTTGTGGTT
TGAACCGTGGCCGTTTGGTTCCACGATCCACTGTGTAATAAAATTTTATCAAGGCAAACA
AAAAACAATTTATGAAAAAGAGAGTTATGATTTTGTGTGAATTATTAAAGATTGGTGGTC
TCATGGCTTCTATTCGTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 12-K115370-0022-301-C06C-8409
GenBank Accession LN491711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115370-0022-301-C06C-8409
AACGAAAGAAGCCATGAGACCACCAATCTTTAATAATTCACACAAAATCATAACTCTCTT
TTTCATAAATTGTTTTTTGTTTGCCTTGATAAAATTTTATTACACAGTGGATCGTGGAAC
CAAACGGCCACGGTTCAAACCACAAAGGAGATTGAAGAAGGGACGTTTAGGCCTCCTATG
ACCGTTACTAACGCCGTTAACAGTAGTTACCCTCTGGACGCTCGTAGTGTTACCAACTCG
TATAGGAGTGACAGTGAGAGAGGCAGGGTCAGCGGTTCGAAGGTCAAGCCAATTGATGAT
GTCAGCAAAAACAAGGTCCACGTTACAGTCAGGCTCACCTGACGTCAGAGCGTGCCACAT
CCCTGGATACAACTTGAGCGTCTTGTCCCTTGTGCTAGCTTTCTCGTACAGAGCTTTGCT
TACCTCTGGATCCGTCACTGTATCTGCTTCTCCGTGTAATACAAAGAATGGCATTGTTAT
CTGCAATATCATGTTGTCTTGGTCAGAAATAATGTCATAACACGGGATCAAGAACAATCA
ATAAGAGAC

Last Updated on 06.12.2018 16:11