Sequences from confirmation amplicon


Confirmation sequences for line 744D02 and hit At3g01020 are shown.
Line ID 744D02
Locus At3g01020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5848
Primers used for PCR CR74 - 8474, show primer details
   
Sequence Name 19-K107428-0022-744-D02C-CR74
GenBank Accession LN494786 [GenBank]
Sequencing primer gene specific primer, CR74: 5'-ATGTAATCGACCACTTTGAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K107428-0022-744-D02C-CR74
CGTTGGCACGGGATTGGTAGGAGCTCCTGCCTGCGGAGACCTCATGAGCCTCCAGATTAA
GGTCGATGATTCAGGTCAGATCATTGATACTCGATTCAAGACCTTTGGCTGTGGCTCCGC
TATCGCCTCATCTTCCGTTGGTATGCCTTCGAATCAGGGTTTTCAATTTTGATCGATTCG
TTAAGAGGCTCATGATCAGAGTCAGGTAGATGTAGACAGGTTATAATTTGTATATTTGTT
TGGTTCAGCTTCTGAATGGATCAAGGGCAAAACCTTAGACGAAGTTATGACCATCAAGAA
CGCGTAAGTGCCTTTTAACCATCAAAATCATGTCTCTTAGATATATCGTCAGGATTGTTA
TCTCTAACTCTGTTCTAGTGCTTGGAATATCCCAAATCTAGTGATATGGTTAACATTTTG
ATGAATGAGTCATTGTTTTGGGATTCTTAAATTTGCAGGGAAATCGCTAAGCATCTTAGG
TTGCCACCGGTAAAGCTACATTGCAGTATGCTTGCAGAGGACGCTATCAAGTCCGCGGTC
AGAGACTACAAGGAGAAGCAACCACAGGTATAAAATCGACAATAACCAATATGAGTAAAT
GCATCATACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 03-K107427-0022-744-D02C-8409
GenBank Accession LN487512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K107427-0022-744-D02C-8409
GCCATTTACATTGAATATGTATGATGCATTTACTCATATTGGTTATTGTCGATTTTATAC
CTGTGGTTGCTTCTCCTTGTAGTCTCTGACCGCGGACTTGATAGCGTCCTCTGCAAGCAT
ACTGCAATGTAGCTTTACCGGTGGCAACCTAAGATGCTTAGCGATTTCCCTGCAAATTTA
AGAATCCCAAAACAATGACTCATTCATCAAAATGTTAACCATATCACTAGATTTGGGATA
TTCCAAGCACTAGAACAGAGTTAGAGATAACAATCCTGACGATATATCTAAGAGACATGA
TTTTGATGGTTAAAAGGCACTTACGCGTTCTTGATGGTCATAACTTCGTCTAAGGTTTTG
CCCTTGATCCATTCAGAAGCTGAACCAAACAAATATACAAATTATAACCTGTCTACATCT
ACCTGACTCTGATCATGAGCCTCTTAACGAATCGATCAAAATTGAAAACCCTGATTCGAA
GGCATACCAACGGAAGATGAGGCGATAGCGGAGCCACAGCCAAAGGTCTTGAATCGAGTA
TCAATGATCTGACCTGAATCATCGACCTTAATCTGGAGGCTCATGAGGTCTCCGCAGGCA
GGAGCTCCTACCAATCCCGTGCCAACGTTAGGGTCATTCCTGTTGAAGGAGCCAACGTTT
CTAGGGTTTTCAAAGTGTCNGAATTAC

Last Updated on 06.12.2018 16:11