Sequences from confirmation amplicon


Confirmation sequences for line 410B05 and hit At3g01435 are shown.
Line ID 410B05
Locus At3g01435 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:167024
Primers used for PCR JG74 - 8474, show primer details
   
Sequence Name 78-K034070-022-410-B05C-JG74
GenBank Accession LN513638 [GenBank]
Sequencing primer gene specific primer, JG74: 5'-ATCTTTTCTCGAGTGTTGATCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K034070-022-410-B05C-JG74
TTCATTACATTATCCTGATCAAAGTTAAGGGCTTTGTTTAGAATTTTGATTTTGGTTTTA
GTCTTCAAGATGGCCGCTGCTTCTCGTTTTGTGATTCTTCTGTGACTAGTTAACATATGG
GTTCATGTATCACAATCTCTAGGTTTGTATTCCTTTCCTGTTTCTTAGGATTTGACATTG
ATCCTTTATTCTTAGGCTTAAGGTTTATGAGTGATGGATGCTGATATTTTCATATTGGAT
GGAATCTCACTGTTTAAAGAACATTTGGGCTCTCATTTGACACATGTCTAGATTAGGGAT
CATTACATTCTGTGAACAAGTAAAAAGCTTTTGATTCTGCTTTTTTTCTAGAGATAATCA
TAAAGTTTGAAAATATCAGCTTCATTGTCTGGTTCATGAACTTGTTGATCTTTGACTGTA
CATAAATGTGCTATAATGTTATCGCCAATCCATAATAAGATGAAGTTCTTGTGAAACTTG
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCACTCCCAAAATGG
   
Sequence Name 62-K034070-022-410-B05C-8409
GenBank Accession LN510685 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K034070-022-410-B05C-8409
AAGCCATTTACAATTGAATATATCCTGCAAGTTTCACAAGAACTTCATCTTATTATGGAT
TGGCGATAACATTATAGCACATTTATGTACAGTCAAAGATCAACAAGTTCATGAACCAGA
CAATGAAGCTGATATCTTCAAACTTTATGATTATCTCTAGAAAAAAAGCAGAATCAAAAG
CTTTTTACTTGTTCACAGAATGTAATGATCCCTAATCTAGACATGTGTCAAATGAGAGCC
CAAATGTTCTTTAAACAGTGAGATTCCATCCAATATGAAAATATCAGCATCCATCACTCA
TAAACCTTAAGCCTAAGAATAAAGGATCAATGTCAAATCCTAAGAAACAGGAAAGGAATA
CAAACCTAGAGATTGTGATACATGAACCCATATGTTAACTAGTCACAGAAGAATCACAAA
ACGAGAAGCAGCGGCCATCTTGAAGACTAAAACCAAAATCAAAATTCTAAACAAAGCCCT
TAACTTTGATCAGGAAATGTAATGAACCCTAATATAGACTTGTGTCAAATTAGCCCAAAC
GATCAACACTCGAGAAAAAGATAAAAGTATCGTTATATCTCAACATCGGATCTGACATGT
GGCATGTTGAGGAACATGACATGGTCGGAGGAGGATACA

Last Updated on 06.12.2018 16:11