Sequences from confirmation amplicon


Confirmation sequences for line 015E12 and hit At3g03250 are shown.
Line ID 015E12
Locus At3g03250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:749980
Primers used for PCR KC79 - 8474, show primer details
   
Sequence Name 32-K103231-0022-015-E12C-8409
GenBank Accession LN500542 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103231-0022-015-E12C-8409
TTTCATTGAAAAACCATATTGTTTAACAAGCTGTTTTCCGACAAATTTATTAGTTTTTCA
ACACATAATCAAACGGTCAACCATAATTGTGGCGAAAAAGAAAACATCCGGAGTTTGAAC
ATAACCATGATTCGTTACATTACCTTGTTCTCGACCACGGCCCTGTCCGGAATTTCAAGC
TTCACACCGGATTTTGCCGCCACAGTCACCTTGCCCTTTAAATCCAAAAGAAACCATTCT
GAGCATATATAAATATCAACACCACCTTGTGAACTAATGAACAATTTACATATATACCTT
GAGAACAATGGAAGAGCCAAACCAGACATCACCAGACACCTTAAGGCTGTCGAGCTCGAC
TATACTAGGAATGGACTTAAACCGGCTCAAGAAAGTAGCCACCTGTTAGACAGACACACT
AAGTGAGGCTCAAGAGTCAAGATAGAAATGACAAATGGGACTTGTCAGGAAATCATTTTC
TTCATTTACCTTCTTGAACTCGGGTCCCAATTCAATTGACGGGTTCGAGGGGTTAGTTCT
AGCT
   
Sequence Name 80-K103232-0022-015-E12C-KC79
GenBank Accession LN513938 [GenBank]
Sequencing primer gene specific primer, KC79: 5'-AAAGCTAGAACTAACCCCTCGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K103232-0022-015-E12C-KC79
ATGGGACGAGTTCAGAAGTAATGAAGAAATGATTTCCTGACAAGTCCCATTTGTCATTTC
TATCTTGACTCTTGAGCCTCACTTAGTGTGTCTGTCTAACAGGTGGCTACTTTCTTGAGC
CGGTTTAAGTCCATTCCTAGTATAGTCGAGCTCGACAGCCTTAAGGTGTCTGGTGATGTC
TGGTTTGGCTCTTCCATTGTTCTCAAGGTATATATGTAAATTGTTCATTAGTTCACAAGG
TGGTGTTGATATTTATATATGCTCAGAATGGTTTCTTTTGGATTTAAAGGGCAAGGTGAC
TGTGGCGGCAAAATCCGGTGTGAAGCTTGAAATTCCGGACAGGGCCGTGGTCGAGAACAA
GGTAATGTAACGAATCATGGTTATGTTCAAACTCCGGATGTTTTCTTTTTCGCCACAATT
ATGGTTGACCGTTTGATTATGTGTTGAAAAACTAATAAATTTATCGGAAAACAGCTTGTT
TAAACAATATGGTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCACGTCCAA

Last Updated on 06.12.2018 16:11