Sequences from confirmation amplicon


Confirmation sequences for line 196F01 and hit At3g04530 are shown.
Line ID 196F01
Locus At3g04530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1221253
Primers used for PCR 69zh - 8474, show primer details
   
Sequence Name 62-K125938-0022-196-F01C-69zh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 69zh: 5'-ATCAACCTTCTCGTCGTACTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K125938-0022-196-F01C-69zh
TTCCCCAACTCCCTCCGCCGTTTCTCCGCCTAACCACACCGCTGATCCGAAATCACAGAG
CTTAACACCGCCGCTTACGAGATCGACTAAGACGTTGTCAGGTTTCACATCCCGATGAAC
GACGTCGCACCGGTGACAATGAGCAAGCGCACTGAGGATCTGTTTCGCGTATGAGGCGGA
TTCTGATTCTGATAACCTCCCCCCGGCGGAGATTAACCGATCGTAAATCGTCATCGGCGG
ATCGACTAATTCCATGACGATCGCAAGAGAATCCTCTGTTTCGTAGAGATCGAAGATCCT
GATGATATTCGGATGCGGCGGTAACATCGCCATGATCCTCGGCTCCGTCTCTATACACTC
GCGATCTAATGCGTCGATGAGCACGCGCTTGTCAATGGTTTTGCAGGCGTAGAATTCTTT
AGTCGCGGGTGAGAAACAGCGAGTGATTGTTCCGAATCTACCTCTGCCGATCTCGTCGCA
AAGCTGGTAGTTGTTTTCCAGTTCGAATTCTCTGGTCATCGGAATATTCCTTTTTTCTTG
TTGTTGTTAGTTTCTTCGTATCTCTGATTCGTGAGAGAGAGAGAGGTTTATATAGAGGAA
TAGAATCAAAGTACTAAAAAAGAGAATATTCCGTTTAAGGAGGTTAAACAGCATTCTGTT
TCAAAAGTCAAGCTTCGAGAACAAAAACTAAGCAAGCAACCGACTCCATTAACTAACGGC
TGCCGTTAGTTACACTGCCATTGCATATTAATTAATTGGAATATTAAATTATCGGAGGTA
AAACCTTTAATGTGCATTACATATGATCTGCACATGTAAATGGCTTTAATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGTTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAATGTAGATGTC
   
Sequence Name 59-K125937-0022-196-F01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K125937-0022-196-F01C-8409
TTACATTGAATATTAAAGCCATTTACATGTGCAGATCATATGTAATGCACATTAAAGGTT
TTACCTCCGATAATTTAATATTCCAATTAATTAATATGCAATGGCAGTGTAACTAACGGC
AGCCGTTAGTTAATGGAGTCGGTTGCTTGCTTAGTTTTTGTTCTCGAAGCTTGACTTTTG
AAACAGAATGCTGTTTAACCTCCTTAAACGGAATATTCTCTTTTTTAGTACTTTGATTCT
ATTCCTCTATATAAACCTCTCTCTCTCTCACGAATCAGAGATACGAAGAAACTAACAACA
ACAAGAAAAAAGGAATATTCCGATGACCAGAGAATTCGAACTGGAAAACAACTACCAGCT
TTGCGACGAGATCGGCAGAGGTAGATTCGGAACAATCACTCGCTGTTTCTCACCCGCGAC
TAAAGAATTCTACGCCTGCAAAACCATTGACAAGCGCGTGCTCATCGACGCATTAGATCG
CGAGTGTATAGAGACGGAGCCGAGGATCATGGCGATGTTACCGCCGCATCCGAATATCAT
CAGGATCTTCGATCTCTACGAAACAGAGGATTCTCTTGCGATCGTCATGGAATTAGTCGA
TCCGCCGATGACGATTTACGATCGGTTAATCTCCGCCGGGGGGAGGTTATCAGAATCAGA
ATCCGCCTCATACGCGAAACAGATCCTCAGTGCGCTTGCTCATTGTCACCGGTGCGACGT
CGTTCATCGGGATGTGAAACCTGACAACGTCTTAGTCGATCTCGTAAGCGGCGGTGTTAA
GCTCTGTGATTTCGGATCAGCGGTGTGGTTAGGCGGAGAAACGGCGGAGGGAGTTGTGGG
AACACCGTATTACGTGGCGCCGGAGGTAGTGATGGGGAGGAAGTA

Last Updated on 06.12.2018 16:11