Sequences from confirmation amplicon


Confirmation sequences for line 036G12 and hit At3g04660 are shown.
Line ID 036G12
Locus At3g04660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1265743
Primers used for PCR KM02 - 8474, show primer details
   
Sequence Name 59-K032606-022-036-G12C-8409
GenBank Accession LN509904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K032606-022-036-G12C-8409
CCTTACAGCCATTTTTGACCACTATATCTGTAATAGAAGGAACTATGGCAGCAACTTTTG
ACCACTCTTCTTTGCTGACATCTTCAAGAACCCACAGGTCCATTGGACCGCTGTATGCCT
GACTCACTATAGCTATCTTTCCACTATGGTCCACCATATTACCAAACTGTTGTAATATAT
AATCCTCAGGTAGCTTAGTAACATTAAATTCTTCAGAGCTCAGATCAAAGCTCATCAGAG
ATCGTTTATCGTTGAAAGAAGCTAAATAATACATCACCCCATCTCTACATATCCCTTGAG
ATCCAGAATGATGACGATGTAGATACTTACATTCGAGCATTCTCCATTTTTGTTTGGGTC
CTAGCGTGAAAACTTGATGCTCCTCTGTCATAGGCTCCTCCCACATAGGATCTTCCCATC
TAAAATAATGTGGTCCACGTAGAGTTCTTACCGTCATACACAAGACTTTGTACAAATCAT
TAACTGGATCATATCCAAAAATGGAGAAAATGTGCTTTCTCTGCGTTT
   
Sequence Name 59-K032606-022-036-G12C-KM02
GenBank Accession LN509905 [GenBank]
Sequencing primer gene specific primer, KM02: 5'-CCTTTACCTAGAATCAAAACGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K032606-022-036-G12C-KM02
TTTTGGAATGATCCAGTTAATGATTTGTACAAGTCTTGTGTATGACGGTAAGAACTCTAC
GTGGACCACATTATTTTAGATGGGAAGATCCTATGTGGGAGGAGCCTATGACAGAGGAGC
ATCAAGTTTTCACGCTAGGACCCAAACAAAAATGGAGAATGCTCGAATGTAAGTATCTAC
ATCGTCATCATTCTGGATCTCAAGGGATATGTAGAGATGGGGTGATGTATTATTTAGCTT
CTTTCAACGATAAACGATCTCTGATGAGCTTTGATCTGAGCTCTGAAGAATTTAATGTTA
CTAAGCTACCTGAGGATTATATATTACAACAGTTTGGTAATATGGTGGACCATAGTGGAA
AGATAGCTATAGTGAGTCAGGCATACAGCGGTCCAATGGACCTGTGGGTTCTTGAAGATG
TCAGCAAAGAAGAGTGGTCAAAAGTTGCTGCCATAGTTCCTTCTATTACAGATATAGTGG
TCAAAAATGGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTATTTC
GACGTGTCTAC

Last Updated on 06.12.2018 16:11