Sequences from confirmation amplicon


Confirmation sequences for line 287F02 and hit At3g05030 are shown.
Line ID 287F02
Locus At3g05030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1396084
Primers used for PCR NE30 - 8474, show primer details
   
Sequence Name 60-K102433-0022-287-F02C-8409
GenBank Accession LN510221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K102433-0022-287-F02C-8409
GCCATTTACAATTGAAGCCATTATACCCACGTCCTATTCTGCAGTCACAATCACAAGTAC
TTCCAAGCTGATATTTGAAAGCATATTCATAGAATCAGCAATAATGCATATATGTTATAA
AAACTAATGTAGTATAACATTTTCCCAGTAAGATCTCTGATAATAAACTGATTTTTCCCC
TCTGTAGTACACAACCCAACCAAACATATTACTAGAACACAAGATGTATCAAATAGAGAA
AGACCAAAAATATGCAACTATCTATCATCAAATGATCAGTCCGGATGTAGAAACTTACCC
TGCATTGAATATTATGGGTGGCAAAAGATATATAAAGAAGAGATCTTCACTAAAGACCAA
GAGATGTGAGTTTTTCCCTCTACTAATCAACAATATGACGACACCAGTGCCAAGCCCCTG
CAACCCAATTGCTTAAAAAGTCAGTAAAGAAAAATGAAAGTGAAACATTGATATGATAAT
ATCCAAAAATGTAAAACTTATCCGTCTCCTCTTTAGCTACCATTCAACGCAAAATAATGG
AAACTTACAATCAATAAAGCAGTGATGGATTCGTTCATCCATCGATTCTCCTCCAAAAGA
TGGCCAATGACGATACAAGCACATAGAAGGGCAACAAA
   
Sequence Name 60-K102434-0022-287-F02C-NE30
GenBank Accession LN510222 [GenBank]
Sequencing primer gene specific primer, NE30: 5'-CACTTAATCTCTTTGTTGCCCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K102434-0022-287-F02C-NE30
TTTTGGAGAGATCGATGGATGAACGAATCCATCACTGCTTTATTGATTGTAAGTTTCCAT
TATTTTGCGTTGAATGGTAGCTAAAGAGGAGACGGATAAGTTTTACATTTTTGGATATTA
TCATATCAATGTTTCACTTTCATTTTTCTTTACTGACTTTTTAAGCAATTGGGTTGCAGG
GGCTTGGCACTGGTGTCGTCATATTGTTGATTAGTAGAGGGAAAAACTCACATCTCTTGG
TCTTTAGTGAAGATCTCTTCTTTATATATCTTTTGCCACCCATAATATTCAATGCAGGGT
AAGTTTCTACATCCGGACTGATCATTTGATGATAGATAGTTGCATATTTTTGGTCTTTCT
CTATTTGATACATCTTGTGTTCTAGTAATATGTTTGGTTGGGTTGTGTACTACAGAGGGG
AAAAATCAGTTTATTATCAGAGATCTTACTGGGAAAATGTTATACTACATTAGTTTTTAT
AACATATATGCATTATTGCTGATTCTATGAATATGCTTTCAAATATCAGCTTGGAAGTAC
TTGTGATTGTGACTGCAGAATAGGACGTGGGTATAATGGCTTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11