Sequences from confirmation amplicon


Confirmation sequences for line 006A01 and hit At3g05290 are shown.
Line ID 006A01
Locus At3g05290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1507714
Primers used for PCR NA21 - 8474, show primer details
   
Sequence Name 03-K101562-0022-006-A01C-8409
GenBank Accession LN487422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101562-0022-006-A01C-8409
CACGTAAGACATTTGCCTCGGGAGTTTAAAAAGACGTCAATATTGACGGCGTTAACATCG
CCGTTATGCTGAGATTTGATGCTTCTCTCTCTATTTGGTACACAGACATTGGTTTAAAGT
TTAAACCGTATAAAATGATAAAATTACAAGAAGAAAGTTGATATATATGGCATTGCAAGA
AGAAAGTATATTACATACATACATACAATTTCTAAATGGACGAAAGTAAACACAAGCCAA
CATTAACAGTAACTGATAAAGAGGGTTTAAGCAAATACATTTCTGATGAAGAAAAGAAAG
AAGATAAAAAGAGCTTACGAGTTACGATAAACTGAACTTCCCCTTTGTATTCTGAAACAC
TTGCTCGGCGATACTCAGATCGAGTTTTGTAATCTCTAGGTTAGAGTTATGGTAGCACCC
AACGCCTCTCACACCATCTGCTAAGATCCTATTGAACCCAATGGCTAAAGCTGCTTCGAC
TTTCGCCTCGTACACCAATCTCTGTTTACAAAGTAATCAAGACGAGGTTCAAGGAAAGA
   
Sequence Name 03-K101571-0022-006-A01C-NA21
GenBank Accession LN487423 [GenBank]
Sequencing primer gene specific primer, NA21: 5'-TTCTTTCTGAAACCTCGTCTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101571-0022-006-A01C-NA21
CGAAGTCGAGCAGCTTTGCCATTGGGTTCAATAGGATCTTAGCAGATGGTGTGAGAGGCG
TTGGGTGCTACCATAACTCTAACCTAGAGATTACAAAACTCGATCTGAGTATCGCCGAGC
AAGTGTTTCAGAATACAAAGGGGAAGTTCAGTTTATCGTAACTCGTAAGCTCTTTTTATC
TTCTTTCTTTTCTTCATCAGAAATGTATTTGCTTAAACCCTCTTTATCAGTTACTGTTAA
TGTTGGCTTGTGTTTACTTTCGTCCATTTAGAAATTGTATGTATGTATGTAATATACTTT
CTTCTTGCAATGCCATATATATCAACTTTCTTCTTGTAATTTTATCATTTTATACGGTTT
AAACTTTAAACCAATGTCTGTGTACCAAATAGAGAGAGAAGCATCAAATCTCAGCATAAC
GGCGATGTTAACGCCGTCAATATTGACGTCTTTTTAAACTCCCGAGGCAAATTGTCTTTA
CGTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11