Sequences from confirmation amplicon


Confirmation sequences for line 014C12 and hit At3g05540 are shown.
Line ID 014C12
Locus At3g05540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1607413
Primers used for PCR BH15 - 8474, show primer details
   
Sequence Name 02-K104151-0022-014-C12C-BH15
GenBank Accession LN487009 [GenBank]
Sequencing primer gene specific primer, BH15: 5'-ATGTTATGGGAAGTTGAAGGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104151-0022-014-C12C-BH15
TAGCTTTAGTTTTTTTTCTTACGATCAAGCTGGTTGAATTAAATTGATGTATCATTGTTA
TTCTTTTTTGTGTTCATTAGTGGGTTGTGAAAGGAGCTATGGATTTTGACATAGGTGCGA
ATCTGTTGATTTTGTAATCAAACAATGAATTTGTACTAAGGGATTTAGACAAATTCACTC
CTCTCTTAGCTCACAAATAATCAAAGGGAAAGAACAAAGTTGTTTGAACAGAAAGCTAGG
GTTTGCTCTATAATCATCTGATTATTAACTATCAATTGAATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCA
   
Sequence Name 02-K104152-0022-014-C12C-8409
GenBank Accession LN487010 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104152-0022-014-C12C-8409
GCCATTTACATTGAATAATTCAATTGATAGTTAATAATCAGATGATTATAGAGCAAACCC
TAGCTTTCTGTTCAAACAACTTTGTTCTTTCCCTTTGATTATTTGTGAGCTAAGAGAGGA
GTGAATTTGTCTAAATCCCTTAGTACAAATTCATTGTTTGATTACAAAATCAACAGATTC
GCACCTATGTCAAAATCCATAGCTCCTTTCACAACCCACTAATGAACACAAAAAAGAATA
ACAATGATACATCAATTTAAATTCAACCAGCTTTGATCGTAAGAAAAAAAAACTCAAAGC
TTTATTTAAGAATAATACCTTTCCTTCAACTTCCCATAAAAT

Last Updated on 06.12.2018 16:11