Sequences from confirmation amplicon


Confirmation sequences for line 531E02 and hit At3g06340 are shown.
Line ID 531E02
Locus At3g06340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1921693
Primers used for PCR KP17 - 8474, show primer details
   
Sequence Name 64-K030601-022-531-E02C-KP17
GenBank Accession LN511116 [GenBank]
Sequencing primer gene specific primer, KP17: 5'-TGCTTTCACTCACCTTAAAACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K030601-022-531-E02C-KP17
GTATGATATGATCGGGCACATGTCAACAAGGAAGTCACTTGTGAGACTTGTAAAAAACNG
TTTCACTGGCTTTTGAAGAACCTTTGCAGAGTGCACCACAGGCGAAAGGCCCATCTCAGA
CTACTTATTGCTTCCCACAGCAGAGCAAATTCCCAGACCAAAGGGCTTGCAGTGAACCAC
ACAAGCGTCCAGAAAATCCTCCCACTGTTTCCTCTAGCAAGGCGAGTTTCCCTATGCCGG
GTTCTACTGCAAAACACAATGGGAAGAGGAAGAGGAAGAATGTGGCTGAATGTAGTGAAA
GCTCTGACAGTGAAAGTAGCAGTGAGTCAATGAGTATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 25-K030601-022-531-E02C-8409
GenBank Accession LN497317 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K030601-022-531-E02C-8409
GCAGTAGAACCCGGCATAGGGAAAACTCGCCTTGCTAGAGGAAAACAGTGGGTGGATTTT
TCTGGACGCTTGTGTGGTTCACTGCAAGCCCTTTGGTCTGGGAATTTGCTCTGCTGTGGG
AAGCAATAAGTAGTCTGAGATGGGCCTTTCGCCTGTGGTGCACTCTGCAAAGGTTCTTCA
AAAGCAGTGAAACGTTTTTTACAAGTCTCACAAGTGACTTCCTTGTTGACATGTGCCCGA
TCATATTCATACCTCACCCGGCAGAAAACACAAGAGGTCCCAAAGGTTTT

Last Updated on 06.12.2018 16:11