Sequences from confirmation amplicon


Confirmation sequences for line 654D09 and hit At3g06370 are shown.
Line ID 654D09
Locus At3g06370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1932228
Primers used for PCR NS32 - 8474, show primer details
   
Sequence Name 65-K102435-0022-654-D09C-8409
GenBank Accession LN511366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K102435-0022-654-D09C-8409
ACAAGCAAAGGATTATTAAAGAAAGGGTAAAATCACATTTTTAACCATCAGTAGATACGA
ATATACCTTCCTATATAGAGCTTCTTGATAACAAAAGCACTGAGCAATCCAGCCTAAAAG
AAAACGAGTGAGAATTTTTCGGGTTTAGAAATTTGAGGAGCGGAGAGAAAGAAACTTACT
GCAACACCAAGTGCTGTGCTTAAGATAAAGAGGTAAAAAAAGTTTCCAGCAAACTCCAAA
GCTATGGCTGAATTGATATTTGTGAGGTCGAATCTCTGTATTGCATTGAAGAGCACGACC
GATGTGGCATCGTTCACTACACCTTCTCCAAAGACAAGACTGTACAAGAGAGGTGTCTCG
TCTTGATTAAGCACCTAGATTTAACAAAGCGTGCAAGTT
   
Sequence Name 65-K102436-0022-654-D09C-NS32
GenBank Accession LN511367 [GenBank]
Sequencing primer gene specific primer, NS32: 5'-ATCAAAAATTAACTTGCACGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K102436-0022-654-D09C-NS32
CCTCTCTTGTACAGTCTTGTCTTTGGAGAAGGTGTAGTGAACGATGCCACATCGGTCGTG
CTCTTCAATGCAATACAGAGATTCGACCTCACAAATATCAATTCAGCCATAGCTTTGGAG
TTTGCTGGAAACTTTTTTTACCTCTTTATCTTAAGCACAGCACTTGGTGTTGCAGTAAGT
TTCTTTCTCTCCGCTCCTCAAATTTCTAAACCCGAAAAATTCTCACTCGTTTTCTTTTAG
GCTGGATTGCTCAGTGCTTTTGTTATCAAGAAGCTCTATATAGGAAGGTATATTCGTATC
TACTGATGGTTAAAAATGTGATTTTACCCTTTCTTTAATAATCCTTTTGCTTTGTTTTTG
TTTTGTTTTGTAACAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11