Sequences from confirmation amplicon


Confirmation sequences for line 953B07 and hit At3g07320 are shown.
Line ID 953B07
Locus At3g07320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2332720
Primers used for PCR 05ug - 8474, show primer details
   
Sequence Name 41-K117042-0022-953-B07C-05ug
GenBank Accession LN504228 [GenBank]
Sequencing primer gene specific primer, 05ug: 5'-CAAAAATTTAGCAAACCCAAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117042-0022-953-B07C-05ug
TGACCGGAACAATTTACGAATCGGTTAATTATGATTTGGATTTTGGAATTTAACTTACTT
GGATCTTTGATGGTTTGTGTAGCTAAGCCGTTGAAGGAGCAAGTTCCCCCGATCTTACGA
AATTGGGCCCAATAAGAGCTAAAGGCGTAACTGGCGTGCAAAACGGTCAAATCAGGTTTC
TGACATGGGCCTCCGCGTTGAATCGGGTCACAGGTATTATTCCCCTGTGAGCAAGCGTAC
GATAGAGCATCACCTAGCTGAGTCCAATTCGCTCCTTTACAGCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 11-K117041-0022-953-B07C-8409
GenBank Accession LN491293 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117041-0022-953-B07C-8409
TCATTGAATATATCCTGCTGTAAAGGAGCGAATTGGACTCAGCTAGGTGATGCTCTATCG
TACGCTTGCTCACAGGGGAATAATACCTGTGACCCGATTCAACGCGGAGGCCCATGTCAG
AAACCTGATTTGACCGTTTTGCACGCCAGTTACGCCTTTAGCTCTTATTGGGCCCAATTT
CGTAAGATCGGGGGAACTTGCTCCTTCAACGGCTTAGCTACACAAACCATCAAAGATCCA
AGTAAGTTAAATTCCAAAATCCAAATCATAATTAAACCGATTCGTAAATTAGTTCCGGTT
CAGTGATATCAGGATGTTAAAACCGGTGGTGGATTTTGGGTTTGTAAAATTTTTG

Last Updated on 06.12.2018 16:11