Sequences from confirmation amplicon


Confirmation sequences for line 184C08 and hit At3g08040 are shown.
Line ID 184C08
Locus At3g08040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2566670
Primers used for PCR 15cy - 8474, show primer details
   
Sequence Name 47-K109414-0022-184-C08C-15cy
GenBank Accession LN506219 [GenBank]
Sequencing primer gene specific primer, 15cy: 5'-CCGGATATCTTTGAACCATCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K109414-0022-184-C08C-15cy
TCTATTTGGGTAGAAAGCCAGTGAGGGCAGAGGGCTCCATATTTTAATGACTTAAAAATA
TGCATTATTTATGTGAAACTGATAATATGATAACGGTCTCTCGAACAAAATGTTGAAAAA
CAAACACAAGAACCATAAACAAAAACGAAGAAGGAAGAAGAGATGCAACTCGTTATAAAT
AAACTAAGTCCTAGGAAGATGAAGAGGATGATCGTCCACGCAAGAACCTCCACGGTCCAG
TTCCTGTCGCCATCCTGCATAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 31-K109413-0022-184-C08C-8409
GenBank Accession LN500201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K109413-0022-184-C08C-8409
GCCATTTACAATTGAATTTTGCAGGATGGCGACAGGAACTGGACCGTGGAGGTTCTTGCG
TGGACGATCATCCTCTTCATCTTCCTAGGACTTAGTTTATTTATAACGAGTTGCATCTCT
TCTTCCTTCTTCGTTTTTGTTTATGGTTCTTGTGTTTGTTTTTCAACATTTTGTTCGAGA
GACCGTTATCATATTATCAGTTTCACATAAATAATGCATATTTTTAAGTCATTAAAATAT
GGAGCCCTCTGCCCTCACTGGCTTTTCTACCCAAATAGAATATTCTGTATGAGTATGTTT
CAGCTAGACTAGATGGTTCAAAGATATCCGG

Last Updated on 06.12.2018 16:11