Sequences from confirmation amplicon


Confirmation sequences for line 675G06 and hit At3g08950 are shown.
Line ID 675G06
Locus At3g08950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2729571
Primers used for PCR 21fx - 8474, show primer details
   
Sequence Name 32-K111683-0022-675-G06C-21fx
GenBank Accession LN500638 [GenBank]
Sequencing primer gene specific primer, 21fx: 5'-TGGTGGTTGGAGTTTTGATATGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K111683-0022-675-G06C-21fx
CGGAGATGATTTTGTGAATTCTACGGGAAGAATCACGATGTTGACTCGTTGACAGACGGC
GTTGTAAAAGAGATCCGTCAGTACCGGAAGTAAAGAACCTTCGAAGTACACTTAGGTGAT
TAATATACATTATTCCCATTTTTGGAAGCTCATCATTAATAAGGATTTCTCAGAACACGG
AGCTGCTTGTGTTTCCTTGAAAAAACATCAGTTTTTGTTGTGGCCGAGCATGCACACAAC
GGTAGTATCCATACTATAAGGGCATTAAGGGATCAATTGAGATTATTCCGATTACCCGTG
TCCCATAAATTGATCACACTTCTTCGTATAGAATTTTAAGGCCTAAATTATCGGCAGTGC
TTAAATGTCGAACTACAGTAAATACCATAGTATGGTAATTGCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT
TATTA
   
Sequence Name 16-K111682-0022-675-G06C-8409
GenBank Accession LN493484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K111682-0022-675-G06C-8409
TCATTGAATATATCCTGCAATTACCATACTATGGTATTTACTGTAGTTCGACATTTAAGC
ACTGCCGATAATTTAGGCCTTAAAATTCTATACGAAGAAGTGTGATCAATTTATGGGACA
CGGGTAATCGGAATAATCTCAATTGATCCCTTAATGCCCTTATAGTATGGATACTACCGT
TGTGTGCATGCTCGGCCACAACAAAAACTGATGTTTTTTCAAGGAAACACAAGCAGCTCC
GTGTTCTGAGAAATCCTTATTAATGATGAGCTTCCAAAAATGGGAATAATGTATATTAAT
CACCTAAGTGTACTTCGAAGGTTCTTTACTTCCGGTACTGACGGATCTCTTTTACAACGC
CGTCTGTCAACGAGTCAACATCGTGATTCTTCCCGTAGAATTTCACAAAATTCATCTCCG
GACTCATCAAGTACCTTCACCCACAACATGTTTACATATCAAAACTCCAACCACC

Last Updated on 06.12.2018 16:11