Sequences from confirmation amplicon


Confirmation sequences for line 548G05 and hit At3g10490 are shown.
Line ID 548G05
Locus At3g10490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3267721
Primers used for PCR 17fu - 8474, show primer details
   
Sequence Name 58-K111688-0022-548-G05C-17fu
GenBank Accession LN509743 [GenBank]
Sequencing primer gene specific primer, 17fu: 5'-GTTCTAGGGTTTTCTCAATTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K111688-0022-548-G05C-17fu
GTTGGGCAGATGGTTTGACGAAGAAGAAACCAAGAGATAAGCACAAGATTTGAGGTACCT
TCAATTGCAAGAAAATTTTGTCTTTCTTTTCGCGTTCCCAGAAAGGCAGGCTAGTAGAGA
GCAACAAAAAAGTATTTTGGATTGCGATACCAATTTTCGATTTACATTTCATTATGTCGC
AGTAATAAACCCTAGCAAAATTTTACTGCGTAGCTTTTTGTAGTAAATTATACTTGGGAG
TAAATAATGAAAAGTAATTTAGTAAATTACCAATGGCTTCATGTAAATAAAGAACATGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT
TATT
   
Sequence Name 42-K111684-0022-548-G05C-8409
GenBank Accession LN504506 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K111684-0022-548-G05C-8409
GTTCTTTATTACATGAAGCCATTGGTAATTTACTAAATTACTTTTCATTATTTACTCCCA
AGTATAATTTACTACAAAAAGCTACGCAGTAAAATTTTGCTAGGGTTTATTACTGCGACA
TAATGAAATGTAAATCGAAAATTGGTATCGCAATCCAAAATACTTTTTTGTTGCTCTCTA
CTAGCCTGCCTTTCTGGGAACGCGAAAAGAAAGACAAAATTTTCTTGCAATTGAAGGTAC
CTCAAATCTTGTGCTTATCTCTTTGGTTTCTTCTTCGTCAAACCATCTGCCCAACTTGTG
TTTTTGTTCTTCAGGTGAGGTTTGGTGAAAGGGAAATTGAGAAAACCCTAGAA

Last Updated on 06.12.2018 16:11