Sequences from confirmation amplicon


Confirmation sequences for line 525H04 and hit At3g12070 are shown.
Line ID 525H04
Locus At3g12070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3845627
Primers used for PCR AB11 - 8474, show primer details
   
Sequence Name 45-K024604-022-525-H04C-8409
GenBank Accession LN505394 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K024604-022-525-H04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCAACAGTATAGCTGGGTC
TATCTGAGATGCCTCCGTTATCCATGTCCTATTCAAAGTTTCACTTGAACCTCAGGGAAG
GCTCTAAAAGATGAGACCTTTAAAACCAATAGCAACAATATTGGAGACAAACCTGAGAGT
CCAAGATAAACTTGACAAGCTTTGCTTTCTCGATCCAGTGAACTCTATCGATCATGATTA
AGCTGGATAAGACCCACCAAGAATAGCAAACCTGATAATAGGAATAATCAAAAGCACGCT
AAGAACAATCAGAATCATGTTACGATTTGGAAGAGTTTAACGCTTACATCAGGGAGTTTC
TCAGGTCGTCCATTTAAACCTCCAGACTCGTAGTCTTGTCTTTCACATAACCA
   
Sequence Name 46-K024604-022-525-H04C-AB11
GenBank Accession LN505739 [GenBank]
Sequencing primer gene specific primer, AB11: 5'-TTACTTGGATGGTGGTTATGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K024604-022-525-H04C-AB11
AAGACTACGAGTCTGGAGGTTTAAATGGACGACCTGAGAAACTCCCTGATGTAAGCGTTA
AACTCTTCCAAATCGTAACATGATTCTGATTGTTCTTAGCGTGCTTTTGATTATTCCTAT
TATCAGGTTTGCTATTCTTGGTGGGTCTTATCCAGCTTAATCATGATCGATAGAGTTCAC
TGGATCGAGAAAGCAAAGCTTGTCAAGTTTATCTTGGACTCTCAGGTTTGTCTCCAATAT
TGTTGCTATTGGTTTTAAAGGTCTCATCTTTTAGAGCCTTCCCTGAGGTTCAAGTGAAAC
TTTGAATAGGACATGGATAACGGAGGCATCTCAGATAGACCCAGCTATACTGTTGAATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACA

Last Updated on 06.12.2018 16:11