Sequences from confirmation amplicon


Confirmation sequences for line 130H02 and hit At3g12160 are shown.
Line ID 130H02
Locus At3g12160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3880475
Primers used for PCR ML23 - 3144, show primer details
   
Sequence Name 42-K100820-0022-130-H02C-3144
GenBank Accession LN504388 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K100820-0022-130-H02C-3144
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGAAACAACAAAGGAGA
ATGAATATACACAAATCTCCAGATCACGAATACGAAAGGCGAAGAGTCTCGGATTCGTAT
TGCACGCGTGAAACAGTCGAATCTACCATCAGACGAACACAGAAACTCAGATCACAATGA
ATGGTGGTTAGGCAGAGAAAGCGAAAAATTATTCAGAGATTGAGAGAAAGAGAGGATTAA
CGGCCGATGCGGGACCCTTTACTCTAGGAATTATTCTACTGTGCCATAGATCACCCTTAA
CCCAATTCATCCTGGCCGTTTACTTTATTCGTACCTTATTCACCATTCAATT
   
Sequence Name 42-K100832-0022-130-H02C-ML23
GenBank Accession LN504390 [GenBank]
Sequencing primer gene specific primer, ML23: 5'-AAATTGAACGGTGAATAAGGTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K100832-0022-130-H02C-ML23
ATTGGGTTAGGGTGATCTATGGCACAGTAGAATAATTCCTAGAGTAAAGGGTCCCGCATC
GGCCGTTAATCCTCTCTTTCTCTCAATCTCTGAATAATTTTTCGCTTTCTCTGCCTAACC
ACCATTCATTGTGATCTGAGTTTCTGTGTTCGTCTGATGGTAGATTCGACTGTTTCACGC
GTGCAATACGAATCCGAGACTCTTCGCCTTTCGTATTCGTGATCTGGAGATTTGTGTATA
TTCATTCTCCTTTGTTGTTTCACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGA
TCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTT
GCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAA
Primers used for PCR FK09 - 8474, show primer details
   
Sequence Name 51-K101327-0022-130-H02C-8409
GenBank Accession LN507442 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101327-0022-130-H02C-8409
ATATATAATAGAAGAAGAGAGAGATGTCTAATTTGTATGGAGATTATACCACCATAAAAG
TACGTCTTCAAAGTCGTGTTGATCGGTGATCCTTCCGTTGGCAAAACTCAGCTTCTTGCT
CGGTTCGCTAGGAATGAGTTTAGCGTCGATTCTAAAGCCACCATTGGTGTTGAATTCCAG
ACTAAAACGCTCGTCATTGATAACAAAACCGTCAAAGCTCAGATTTGGGATACTGCTGGC
CAAGAAAGGTTTTGCTTTTTTCTTCTTCATTCATTAGAAGTAATGACACCATGAATATCA
TTTGGTATATATCAGAGAACGTTCACAATTCATCCATTTGATTG
   
Sequence Name 51-K101333-0022-130-H02C-FK09
GenBank Accession LN507443 [GenBank]
Sequencing primer gene specific primer, FK09: 5'-CAATCAAATGGATGAATTGTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101333-0022-130-H02C-FK09
TGGTGTCATTACTTCTATGAATGAAGAAGAAAAAAGCAAAACCTCTCTTGGCCAGCAGTA
TCCCAAATCTGAGCTTTGACGGTTTTGTTATCAATGACGAGCGTTTTAGTCTGGAATTCA
ACACCAATGGTGGCTTTAGAATCGACGCTAAACTCATTCCTAGCGAACCGAGCAAGAAGC
TGAGTTTTGCCAACGGCAGAATCACCGATCAACACGACTTTGAAGACGTAATCGATCTTT
TGGTTATAATCTCCATACAAATTAGACATCTCTCTTCTTCTTCTTATTATTATTATTTAA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11