Sequences from confirmation amplicon


Confirmation sequences for line 366G12 and hit At3g12360 are shown.
Line ID 366G12
Locus At3g12360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3934946
Primers used for PCR KR29 - 8474, show primer details
   
Sequence Name 71-K030473-022-366-G12C-8409
GenBank Accession LN512483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K030473-022-366-G12C-8409
TCCATTTCTCTTCGCCTGAAATTTAGACAAAAACCATAGCTGGGGCAGTAGCAGTTGCAG
TTGGTGAAGGTGTTGGTGTAGGATCTATCATAGGATTTTGATTCTCACTTGACTGTAGTA
ATATCATTCCTCCTTTCTCCATATCTCTTTCTCCTGAAAAAATTTCAGC
   
Sequence Name 72-K030473-022-366-G12C-KR29
GenBank Accession LN512649 [GenBank]
Sequencing primer gene specific primer, KR29: 5'-TGAATCCTGAGGTTTTTGTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K030473-022-366-G12C-KR29
AAATCAAAAATCCTCTGATAGATCCTTTCACCAACACCTTCACCATCTGCAACCGCTACA
GCCCCAGCTTTTGGTTTTGTCTAATTCAGGCAAGAGAAATCGGATCAAGCTCGGCAAGAA
AAAGTATGTTAAGCAAGTCACGGGTCGCCACAACGACACCGAGCTTCACTTAGCGGCCCA
ACGAGGTGATTTGGCTGCTGTGCAGCAGAATTCTCAAGGATATTAACTCTCAGATGGAAG
GGATTCTGAGTGGGGAAGAGTTTGATGCTGAAGTTGCGGAGATTCGAGCATCGATTGTGA
ATGAGGTGAATGAGCATGTAGATTTATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTT

Last Updated on 06.12.2018 16:11