Sequences from confirmation amplicon


Confirmation sequences for line 042E02 and hit At3g12480 are shown.
Line ID 042E02
Locus At3g12480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3958606
Primers used for PCR 01re - 8474, show primer details
   
Sequence Name 74-K115990-0022-042-E02C-01re
GenBank Accession LN513136 [GenBank]
Sequencing primer gene specific primer, 01re: 5'-TTTGATCTGTTGTTTTTCAGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K115990-0022-042-E02C-01re
GATGAAATTAGATTAAGTATCTCATTTCTGGCGAATTAGTTTTAGCAAATGACCTAGTAG
TCAATTTCACCCTAATTTTTGTTGTGCTTGTTATATAGCTATAGTAATATGTGTTATGAA
GATCCGCTATGGAATGTTTCATGTAAATTAGAGAATTCGAGAATTTAGGTGACTAGTGCT
CGAAACGTGTTCTTTATCAACAAACCAGTCAGCTATTGGTTAGATAGTGTTGATTGAAGC
AGGGATGTTATACACGAATTGTTTTTTCATAGGCTTGGAACTTTTTGTAGAGCTTCCAAT
TTGGCTAGCTAGATTTTGTTTAGCTTTATTGAATAAAACGTCAGATGATTTCATAAGTTG
GTTATTGTTGATGGTGGTTTATTTTATTGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 55-K115989-0022-042-E02C-8409
GenBank Accession LN508908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K115989-0022-042-E02C-8409
GCCATTTACATTGAATATATCCAATAAAATAAACCACCATCAACAATAACCAACTTATGA
AATCATCTGACGTTTTATTCAATAAAGCTAAACAAAATCTAGCTAGCCAAATTGGAAGCT
CTACAAAAAGTTCCAAGCCTATGAAAAAACAATTCGTGTATAACATCCCTGCTTCAATCA
ACACTATCTAACCAATAGCTGACTGGTTTGTTGATAAAGAACACGTTTCGAGCACTAGTC
ACCTAAATTCTCGAATTCTCTAATTTACATGAAACATTCCATAGCGGATCTTCATAACAC
ATATTACTATAGCTATATAACAAGCACAACAAAAATTAGGGTGAAATTGACTACTAGGTC
ATTTGCTAAAACTAATTCGCCAGAAATGAGATACTTAATCTAATTTTCATCGTAGAACAC
TAATCTAATCCCAAATAGTGTTGGAAATTCGATCTGAAAAACAAAGGATCAA

Last Updated on 06.12.2018 16:11