Sequences from confirmation amplicon


Confirmation sequences for line 501G07 and hit At3g13222 are shown.
Line ID 501G07
Locus At3g13222 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4252752
Primers used for PCR 07pf - 8474, show primer details
   
Sequence Name 41-K115075-0022-501-G07C-07pf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 07pf: 5'-TGGAGTCATCACACCCAATAGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115075-0022-501-G07C-07pf
TAAGGTGTCCCAACTGGGAAGCTGAACTCAAACCATTGAATATATCGCCGACTTAGGAGG
GTCAATAT
   
Sequence Name 09-K115074-0022-501-G07C-8409
GenBank Accession LN490308 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115074-0022-501-G07C-8409
CATTCTGTATTCTGTTAAGTTGTTCCTGAGTTTGATTACAGCCTCTTATGTGATTCGACG
TATGGCTTTATTTGGAACAGTCATTAAGAGTGAGGTTGCAGCCAGTAAATGCAAGAATGA
GTCGCTTCTCAAGTCGGATGTTGGTGAACGGCCGCACGTAACATTTCCTGTCCACATTCA
GGTTGCCAAAATGCTGGAAAATGGTCTGACATTTGGGAGCTTCGATTCTAATTTTGTGAG
AGAGGCATCTTCTGACAAGTTTACTATTGGGTTGAATTGACTC

Last Updated on 06.12.2018 16:11