Sequences from confirmation amplicon


Confirmation sequences for line 332D11 and hit At3g15730 are shown.
Line ID 332D11
Locus At3g15730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5332502
Primers used for PCR DB03 - 8474, show primer details
   
Sequence Name 33-K026770-022-332-D11C-8409
GenBank Accession LN500851 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K026770-022-332-D11C-8409
GTAGATCTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTAGCACGTCTGATTGC
ATGAATGTAAGCATCTTGGATACTCCTATCAATGATGTTATCTTTCCCACTTACAAGCCC
GGCTTCCGCAGCAGCTTCAGGCGACTCGGGAAACCCAGCAGCAGCTCCTCCATCAATGGA
CCTAAACAATTGGACATTCCACACATCGTGGTCCTCTTGGAACATAACAGGAGAAGGGGT
GATAATAATATCACTAAGATCTCTCAACTTAACCAGAATGTCTTTACCACCCTGCTTGCT
CCATCTCTGCTCGAAGTTGTACATGACATCCCAAGCAATTGGACCTTCAAGACGGGAGTG
AATGTCATGCCAAGGCTCCCTTGGACCACCTTTAGTGATAGCAGCACCAGTGAAGTTAGG
TTGATGGAAGTCATCATGGTGGACTGTGTCCAATGTCCTGAACAAGGAGTGGAACGGAGT
GTCGTATCTTCCATCACAAAGATCAATACCGCCAACAAAACTCAC
   
Sequence Name 34-K026770-022-332-D11C-DB03
GenBank Accession LN501272 [GenBank]
Sequencing primer gene specific primer, DB03: 5'-TGAGGAGAATTGTGAGTTTTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K026770-022-332-D11C-DB03
TATTGATCTTTGTGATGGAAGATACGACACTCCGTTCCACTCCTTGTTCAGGACATTGGA
CACAGTCCACCATGATGACTTCCATCAACCTAACTTCACTGGTGCTGCTATCACTAAAGG
TGGTCCAAGGGAGCCTTGGCATGACATTCACTCCCGTCTTGAAGGTCCAATTGCTTGGGA
TGTCATGTACAACTTCGAGCAGAGATGGAGCAAGCAGGGTGGTAAAGACATTCTGGTTAA
GTTGAGAGATCTTAGTGATATTATTATCACCCCTTCTCCTGTTATGTTCCAAGAGGACCA
CGATGTGTGGAATGTCCAATTGTTTAGGTCCATTGATGGAGGAGCTGCTGCTGGGTTTCC
CGAGTCGCCTGAAGCTGCTGCGGAAGCCGGGCTTGTAAGTGGGAAAGATAACATCATTGA
TAGGAGTATCCAAGATGCTTACATTCATGCAATCAGACGTGCTAAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11