Sequences from confirmation amplicon


Confirmation sequences for line 355F04 and hit At3g16320 are shown.
Line ID 355F04
Locus At3g16320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5533367
Primers used for PCR 18fp - 8474, show primer details
   
Sequence Name 40-K111630-0022-355-F04C-18fp
GenBank Accession LN503748 [GenBank]
Sequencing primer gene specific primer, 18fp: 5'-TTATGCTTTGGAAGGAATGGATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K111630-0022-355-F04C-18fp
TATTTGCAAATTTTCCTATTATTCATTATGAACAGTCCCGAACTAATCTTGAACATGATG
TTGACTCTGAACATTATCCTGTTGTGATATCAGCATAGAGAACTTGATTTTTGATTGACC
TGCAAGGATTTCTATGTAATTGTTTTTTACTTTTAGTAATTTCGTATTTTTGTTTTTGAA
GCACCTGAAAGAAGAGATGAGGTTGGGCTATCTGGCTCAGGAACTGATTTCAGTTGATCG
CCTGTCTCCAGAATCCTGGTATCATTCTTAGAATCTTCATTACCAATATGCTCTTCCGCT
TTCGTTTCGCAAAACTTACATGATCAACCCACATATGTCTACGTCGAGACTCTAACACCA
CAAGCTTTAACCACATACCGTCAATGGAAACCAGCTTTTTGCACTAGCATATCAAAATCT
GAAACTGCAGACTTTTCTCTTTGCTTGTATTTTTTTCTGTAATGACTGTGGTCGCAATAT
GAATTGCAGGTGTGCAGTTGGGAACTGTTACAGTTTGCGTAAGGATCATGATACTGCTCT
CAAAATGTTTCAGAGAGATTCACATATGAGTATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 24-K111629-0022-355-F04C-8409
GenBank Accession LN497050 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111629-0022-355-F04C-8409
CATTTCAATTGAATATATACTCATATGTGAATCTCTCTGAAACATTTTGAGAGCAGTATC
ATGATCCTTACGCAAACTGTAACAGTTCCCAACTGCACACCTGCAATTCATATTGCGACC
ACAGTCATTACAGAAAAAAATACAAGCAAAGAGAAAAGTCTGCAGTTTCAGATTTTGATA
TGCTAGTGCAAAAAGCTGGTTTCCATTGACGGTATGTGGTTAAAGCTTGTGGTGTTAGAG
TCTCGACGTAGACATATGTGGGTTGATCATGTAAGTTTTGCGAAACGAAAGCGGAAGAGC
ATATTGGTAATGAAGATTCTAAGAATGATACCAGGATTCTGGAGACAGGCGATCAACTGA
AATCAGTTCCTGAGCCAGATAGCCCAACCTCATCTCTTCTTTCAGGTGCTTCAAAAACAA
AAATACGAAATTACTAAAAGTAAAAAACAATTACATAGAAATCCTTGCAGGTCAATCAAA
AATCAAGTTCTCTATGCTGATATCACAACAGGATAATGTTCAGAGTCAACATCATGTTCA
AGATTAGTTCGGGACTGTTCATAATGAATAATAGGAAAATTTGCAAATAGTGACAACAGC
TCACATAAAGAACAGTGGAGTATGTATCCATTCCTTCAAAAGCATAA

Last Updated on 06.12.2018 16:11