Sequences from confirmation amplicon


Confirmation sequences for line 178D01 and hit At3g18610 are shown.
Line ID 178D01
Locus At3g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6406279
Primers used for PCR BU51 - 8474, show primer details
   
Sequence Name 04-K104352-0022-178-D01C-BU51
GenBank Accession LN487902 [GenBank]
Sequencing primer gene specific primer, BU51: 5'-GTATGGAGTTTTTACCTGCTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K104352-0022-178-D01C-BU51
CATTTCAATCAGAATCCTGGTTTCTTAAAAGGTTAGAACATATATTATTTATAAACACTC
TATAGAGAAATCTGAGAAGTAACATTATTGGTACCTTTTTCTTTGGTGTCACTTTCTCAT
CTTTACTCTCCTCCTTATCAGATTCATCACTAGATTCATCACTAGAGCTTTCTTGTTTAG
AAGTTTTTGATGAAACTTTAGCCTTCTTCGTAGGAGGTTTTTCATCATCACTTTCCTGAA
AGTAGGAAAATAATATGAAATATTTAGTGTATAGAATATAAAACTAATCACAAGTCATTA
AAAATATTCAATCTTGAGAAATAAAACAAACCTCCTCATCAGAATCTTCCTCTGAGGATG
ATGAATCTTTTGCAGCTGGTTTAGCATTCTTAACAACTGTTGGCTTCTTGGCAGGTGTTG
GTTCCTGATTACGTTTGATAAAAAACAAATCTCAAAGTAAGTAAAATGATTTACTTTACC
ATTGAGTGAATAACATGTATAACGCAATTCTCACATCGTCCGAAGAAGATTCCTCCTCTG
ATGAACTGCTTTCTGCCTTGGCATCCTTAACAAAGAGTAATGAGTATGATGGTCAATATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAN
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 20-K104353-0022-178-D01C-8409
GenBank Accession LN495195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K104353-0022-178-D01C-8409
ATTGACCATCATACTCATTACTCTTTGTTAAGGATGCCAAGGCAGAAAGCAGTTCATCAG
AGGAGGAATCTTCTTCGGACGATGTGAGAATTGCGTTATACATGTTATTCACTCAATGGT
AAAGTAAATCATTTTACTTACTTTGAGATTTGTTTTTTATCAAACGTAATCAGGAACCAA
CACCTGCCAAGAAGCCAACAGTTGTTAAGAATGCTAAACCAGCTGCAAAAGATTCATCAT
CCTCAGAGGAAGATTCTGATGAGGAGGTTTGTTTTATTTCTCAAGATTGAATATTTTTAA
TGACTTGTGATTAGTTTTATATTCTATACACTAAATATTTCATATTATTTTCCTACTTTC
AGGAAAGTGATGATGAAAAACCTCCTACGAAGAAGGCTAAAGTTTCATCAAAAACTTCTA
AACAAGAAAGCTCTAGTGATGAATCTAGTGATGAATCTGATAAGGAGGAGAGTAAAGATG
AGAAAGTGACACCAAAGAAAAAGGTACCAATAATGTTACTTCTCAGATTTCTCTATAGAG
TGTTTATAAATAATATATGTTCTAACCTTTTAATGAAACCAGGATTCTGATGTTAAAATG
GTGGATGCAGAGCAGAAATCTAATGCAAAGCAGGTAAAAACTCCAT

Last Updated on 06.12.2018 16:11