Sequences from confirmation amplicon


Confirmation sequences for line 362G06 and hit At3g19040 are shown.
Line ID 362G06
Locus At3g19040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6575399
Primers used for PCR 08iv - 8474, show primer details
   
Sequence Name 57-K113020-0022-362-G06C-08iv
GenBank Accession LN509473 [GenBank]
Sequencing primer gene specific primer, 08iv: 5'-TTCAAGACAACTGCGAAAATAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113020-0022-362-G06C-08iv
AAGGATCTAGAAAGTTCCAGAGTTCTGATCTCTAGTATTTATGACGCACTGGACTCCAAC
TTAACAAGAGATTATTTTCCCGAGCTAAACAAGTATAAGTGTTGAGAAAACTAAACCAAT
CTGAGATTAATGAAATGACAAAACAAAAGTAGAGAATAGAGTGTGCTAATCGTTTTGTTC
AAGAAACGGAAATTTCAAAAGCAAACCTGGTTCCCTCTCTTCTGAATCAAAAGATTTCTC
AACTGGTACATGTTCCTTCTCTTGTCCTCCTTGTTCATCATAATCTTCGTCGTCAAACAC
AGAAGCTCTGGAATTTAAACCACTCAAAGCAAATGCAGCTGATAAGTACTCTCTTTTTGG
TAGTAGGTGATCTTCCTCAGTAACTACTTGAAGCTCAGGCCCATCATACTCTTCATCATT
GCTCCCATAATCTACACGACATATCATCACAAGTCAGTGAAGCTGATGACCGGGAATTGG
TAGTGGCAAATAGTTTCAGTTTGTAACTGCTATGGGTGGGACTTGTTGCGTATATCAAAA
TCTCAAACATAAAAACCTTCTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 25-K113019-0022-362-G06C-8409
GenBank Accession LN497525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113019-0022-362-G06C-8409
TTGAATAGAAGGTTTTTATGTTTGAGATTTTGATATACGCAACAAGTCCCACCCATAGCA
GTTACAAACTGAAACTATTTGCCACTACCAATTCCCGGTCATCAGCTTCACTGACTTGTG
ATGATATGTCGTGTAGATTATGGGAGCAATGATGAAGAGTATGATGGGCCTGAGCTTCAA
GTAGTTACTGAGGAAGATCACCTACTACCAAAAAGAGAGTACTTATCAGCTGCATTTGCT
TTGAGTGGTTTAAATTCCAGAGCTTCTGTGTTTGACGACGAAGATTATGATGAACAAGGA
GGACAAGAGAAGGAACATGTACCAGTTGAGAAATCTTTTGATTCAGAAGAGAGGGAACCA
GGTTTGCTTTTGAAATTTCCGTTTCTTGAACAAAACGATTAGCACACTCTATTCTCTACT
TTTGTTTTGTCATTTCATTAATCTCAGATTGGTTTAGTTTTCTCAACACTTATACTTGTT
TAGCTCGGGAAAATAATCTCTTGTTAAGTTGGAGTCCAGTGCGTCATAAATACTAGAGAT
CAGAACTCTTGGAACTTTTCTTAGATTCCTTTTGTGTTAGTGATTATGAGACACTAAACA
TTATTTTC

Last Updated on 06.12.2018 16:11