Sequences from confirmation amplicon


Confirmation sequences for line 839D03 and hit At3g19150 are shown.
Line ID 839D03
Locus At3g19150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6617770
Primers used for PCR BW28 - 8474, show primer details
   
Sequence Name 12-K104337-0022-839-D03C-BW28
GenBank Accession LN491558 [GenBank]
Sequencing primer gene specific primer, BW28: 5'-CTTCTAGACATGTTCCGACAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104337-0022-839-D03C-BW28
GTAGTTATCGATGTATCTTCTGCTATAGGTATCTCTAATTTATTGTTCCGACCACCACAA
TTCTTTTCTAAGTATTTTATAAATTGGTTAACCATGTATCTTCTGTTGTGTAATTATAGC
TAGTATCTTCATTTGAAATATTGTAAGGAATAGTTTGTCTAATTGCTTATGATATTTGAC
CATCTAAGTAAGTTTATGAACTAATTGTTTTTCAAAAAAGGTTGATTAACCTTAATTACG
TGATAGTTTTAACATTAAAAGATTTCAACACTATGTTCTGGATGTTAACATATCCATCTC
AAAACCATGATAAAACGCGTACAATGGAAGGGATCAAGCTAATTTGACAAATCTCTATTC
ACACCTCAACCATTCATATATATACCCTCGATATATGTCACATCATTGGTTTTAGCTTTT
AATTGATTCTCATAAAATATAAAACCCAATTACGAGAAAAGACTTGTTCGATGGTTCTCA
TAGACCCTGACCAAATAAGACTTGTTCCTAAACAAATCGAATTAAATAGAGTTGTGGTTT
ACTCATACTTTAAATCGTTTTAAGAAAAAATAATGACTACTGTTACGTTGCTACTACGTC
ATAACTACCGAAAATGATTGCGTTTTCAATTGCTTTGAAAGGAAGCCAAAAAGGCAAAAA
CGGCAAAAAGGGAGAGAAAACGCGTTTGGGAATCTCAACGGCGTTTGTGTAAGAAAATAA
AAGCGAAACTCCGTAACGGCGCCGTTATATACCATAACCTCCGTCCGCTCACCAAGGAAA
TAAAATCTCAACCGTTGAAATAATCAACGGCTCANGATTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGGTCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTTCATTCAAAAATGT
   
Sequence Name 28-K104338-0022-839-D03C-8409
GenBank Accession LN498825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104338-0022-839-D03C-8409
ATCAGAGCCGTTGATATTTCAACGGTTGAGATTTTATTTTCCTTGGTGAGCGGACGGAGG
TTATGGTATATAACGGCGCCGTTACGGAGTTTCGCTTTTATTTTCTTACACAAACGCCGT
TGAGATTCCCAAACGCGTTTTCTCTCCCTTTTTGCCGTTTTTGCCTTTTTGGCTTCCTTT
CAAAGCAATTGAAAACGCAATCATTTTCGGTAGTTATGACGTAGTAGCAACGTAACAGTA
GTCATTATTTTTTCTTAAAACGATTTAAAGTATGAGTAAACCACAACTCTATTTAATTCG
ATTTGTTTAGGAACAAGTCTTATTTGGTCAGGGTCTATGAGAACCATCGAACAAGTCTTT
TCTCGTAATTGGGTTTTATATTTTATGAGAATCAATTAAAAGCTAAAACCAATGATGTGA
CATATATCGAGGGTATATATATGAATGGTTGAGGTGTGAATAGAGATTTGTCAAATTAGC
TTGATCCCTTCCATTGTACGCGTTTTATCATGGTTTTGAGATGGATATGTTAACATCCAG
AACATAGTGTTGAAATCTTTTAATGTTAAAACTATCACGTAATTAAGGTTAATCAACCTT
TTTTGAAAAACAATTAGTTCATAAACTTACTTAGATGGTCAAATATCATAAGCAATTAGA
CAAACTATTCCTTACAATATTTCAAATGAAGATACTAGCTATAATTACACAACAGAAGAT
ACATGGTTAACCAATTTATAAAATACTTAGAAAAGAATTGTGGTGGTCGGAACAATAAAT
AGAGATACCTATAGCAGAAGATACATCGATNAACTACATTCTATAAAATCATCTGTACTA
TTATATAT

Last Updated on 06.12.2018 16:11