Sequences from confirmation amplicon


Confirmation sequences for line 528H09 and hit At3g22231 are shown.
Line ID 528H09
Locus At3g22231 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7846380
Primers used for PCR 79zj - 8474, show primer details
   
Sequence Name 22-K127435-0022-528-H09C-79zj
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 79zj: 5'-TCCTTAATTCCTCACTATCGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K127435-0022-528-H09C-79zj
ATTTGTATGAATCGTTAATTTTGAAGTTAATGAGTCCCATGCAAGGATCATTCACTGTTG
GTCAGAAACAAGATGTAATTAAGAACACTAAAACACAGTTTCCTTTGAGTAGTAAAGAGT
TATGACTAGCTATTTGGTTTTTAATTTCTTTTATTTATATAGAAACAAAATACACGAAGC
AAATGATTCATTTTAAACAGTGAAACAAATAAACTTTAAAATGCGTGGAAATCCATAGAA
ACGACTACTATAACATATATATATATATATATATATATAGATTTTCTATCACGTGTTGTT
TTTCGGGTGTGGGCAACGACTTCTGTCTCATCATGCTGATTTACTCTGATGTACAGAGGC
TGGAGCATACCCCGCAACAAAAGATACACTCCATACAAGTACTGCCAATAGTGTCAACAG
ACATATCAGTAAAAAAAAAAAGTAGACCGCGGGATATAAAAAAAAATAACCTCTGGGATA
ATTAGAGCCAAGTTAAAAACATATATATATATATATATACACGCGCCCAAAAAACCACTT
TTTGGGGTTGGGGGGGACACCCCCTGGGATTTTCTCCCCAGGGGGGGGGGGAGAAGATCC
CCCCCCCCCGTCTCTCTAGAGAGAAAAAAAGGGGGGGGGGGGGGGATATATTCAATTGAA
AGGGGCTTCGTGCGGGGAAAATCTATGGGGATCCCCAGTGTGTGTGGGGCTCTATGTGGA
AAAAAAGAAAGATTTTTCCCCTTTTTTTTTTCAAA
   
Sequence Name 06-K127434-0022-528-H09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K127434-0022-528-H09C-8409
TTACATTGAATATATCCTGCCGCCACCAATTGGTTATCCGACTAGAGATGCGGTGGTGGG
TGATCCTCCGGCAGCAGCAGTGGAGACAAACTCCAAGGGCGTCAACCCCGAAGCCATAAT
GAGTTGTTTTAGGTACGTGTATATATATATATATATATATGTTTATAACTTGGCTCTAAT
TATCCAAGATGTTATTTTTTTATATATCCGGCGTTCTACTTTTTTTTTTTACTGATATGT
CTGTTGACACTATTGGCAGTACTTGTAGGGAGTGTATCTTCTGCTGCGGCGTATGCTCCC
CCCTCTGTACATCAAAATAAATCAGCATGATGAAACAAAAGTCTTTGCCCAAACCTGAAA
AACAACACGTGAAAAAAAATCTATATATATATATATATATATATATGTTATAGGACTCTT
TTCTGGGGATCCCCCCCCTTTAAAAATTTTTTTTTTCTCACTGAAAAAAAAAAATCTTTC
GCTGCGTGTTTTTTTTTTCTATATAAAAAAAAAATATTAAACCCCATAGGGCTGGTCATA
TCTTTTTACCACAAAAAAAAAAGTGTGTTTTAGTGTTCAAAAACACCTCTTGTCACAAAC
AAAAAGAAAAAGATCCTTGTGGGACACTCTTTATCTAAAAAAAAAACTTTTTCAAACAAT
TTTTTGGTGAAAAAAAAAAAAAAAAAATTTGTTTCACGAGAGGGAAAAATAAAAGAACCC
TTTTTTTCGTTTTT

Last Updated on 06.12.2018 16:11