Sequences from confirmation amplicon


Confirmation sequences for line 944B04 and hit At3g23150 are shown.
Line ID 944B04
Locus At3g23150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8255804
Primers used for PCR 21di - 8474, show primer details
   
Sequence Name 72-K109678-0022-944-B04C-21di
GenBank Accession LN512742 [GenBank]
Sequencing primer gene specific primer, 21di: 5'-TTTTCTCCGAAGATGGTTAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K109678-0022-944-B04C-21di
GTTTGTTTCTCCGGTTTAGCTATAACGGCGGTGGTTATCCACGATGTAACTGCGAAGACG
AAGGAAACAGTTTCTGGAGTACAGAGAACATTCTAGAAACTCAAAGAGTAAGCGATTTCT
TAATCGCAGTAGCTTATTTCTCAATCCCTATTGAGTTACTTTACTTCGTGAGTTGTTCCA
ATGTTCCATTCAAATGGGTTCTCTTTGAGTTTATCGCCTTCATTGTTCTTTGTGGTATGA
CTCATCTTCTTCATGGTTGGACTTACTCTGCTCATCCATTTAGATTAATGATGGCGTTTA
CTGTTTTCAAGATGTTGACTGCTTTAGTCTCTTGTGCTACTGCGATTACGCTTATTACTT
TGATTCCTCTGCTTTTGAAAGTTAAAGTTAGAGAGTTTATGCTTAAGAAGAAAGCTCATG
AGCTTGGTCGTGAAGTTGGTTTGATTTTGATTAAGAAAGAGACTGGCTTTCATGTTCGTA
TGCTTACTCAAGAGATTCGTAAGTCTTTGGATCGTCATACGATTCTTTATACTACTTTGG
TTGAGCTTTCGAAGACTTTAGGGTTGCAGAATTGTGCGGTTTGGATGCCGAATGACGGTG
GAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 68-K109677-0022-944-B04C-8409
GenBank Accession LN512035 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K109677-0022-944-B04C-8409
GCCATTCACCGTCATTCGGCATCCAAACCGCACAATTCTGCAACCCTAAAGTCTTCGAAA
GCTCAACCAAAGTAGTATAAAGAATCGTATGACGATCCAAAGACTTACGAATCTCTTGAG
TAAGCATACGAACATGAAAGCCAGTCTCTTTCTTAATCAAAATCAAACCAACTTCACGAC
CAAGCTCATGAGCTTTCTTCTTAAGCATAAACTCTCTAACTTTAACTTTCAAAAGCAGAG
GAATCAAAGTAATAAGCGTAATCGCAGTAGCACAAGAGACTAAAGCAGTCAACATCTTGA
AAACAGTAAACGCCATCATTAATCTAAATGGATGAGCAGAGTAAGTCCAACCATGAAGAA
GATGAGTCATACCACAAAGAACAATGAAGGCGATAAACTCAAAGAGAACCCATTTGAATG
GAACATTGGAACAACTCACGAAGTAAAGTAACTCAATAGGGATTGAGAAATAAGCTACTG
CGATTAAGAAATCGCTTACTCTTTGAGTTTCTAGAATGTTCTCTGTACTCCAGAAACTGT
TTCCTTCGTCTTCGCAGTTACATCGTGGATAACCACCGCCGTTTATAGCTAAAACCGGAG
AAACAAACACCACCATTGATAGTATCAATAACCAAGAAGCTATTTCTTTAACCATCT

Last Updated on 06.12.2018 16:11