Sequences from confirmation amplicon


Confirmation sequences for line 411D06 and hit At3g23550 are shown.
Line ID 411D06
Locus At3g23550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8449013
Primers used for PCR 53um - 8474, show primer details
   
Sequence Name 53-K117497-0022-411-D06C-53um
GenBank Accession LN508280 [GenBank]
Sequencing primer gene specific primer, 53um: 5'-CCTCTCGCAACTCCTGTAAAATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117497-0022-411-D06C-53um
TTCAGAAGAAGAAGATTTTCTTGGATCTTTATACCTGATAAAACACCTTGGATTGAATCA
AGTGTTATAGATGCAGCAAGGAAAAACCTTAGTGATGCAAATCCCTCTTTAATCACATGG
CTATTGCTGAATAAACCAACCCAGGCATCATGACCTACAAGTATAGCAATGACTACACCG
AGAGCTAGAACTAAAGACAACTTAACGGAGACCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTTATTAT
   
Sequence Name 21-K117496-0022-411-D06C-8409
GenBank Accession LN495836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117496-0022-411-D06C-8409
CATTACATTGAATATATCCTGGTCTCCGTTAAGTTGTCTTTAGTTCTAGCTCTCGGTGTA
GTCATTGCTATACTTGTAGGTCATGATGCCTGGGTTGGTTTATTCAGCAATAGCCATGTG
ATTAAAGAGGGATTTGCATCACTAAGGTTTTTCCTTGCTGCATCTATAACACTTGATTCA
ATCCAAGGTGTTTTATCAGGTATAAAGATCCAAGAAAATCTTCTTCTTTCTTGAACTATC
AAGCGCGAAGAAACTCATTTCTTTAATGTATTTTACAGGAGTTGCGAGAGG

Last Updated on 06.12.2018 16:11