Sequences from confirmation amplicon


Confirmation sequences for line 185E01 and hit At3g25795 are shown.
Line ID 185E01
Locus At3g25795 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9418054
Primers used for PCR NB57 - 8474, show primer details
   
Sequence Name 01-K034547-022-185-E01C-8409
GenBank Accession LN486471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K034547-022-185-E01C-8409
TGAGCCATTTCCCGGTCACTTAATTAACAACACTTGTATAAATGAGGTAAGATTGAAAAA
TGGGAAGGATATTAACAACAATTACGTAGAGTTAATTAACTTTACGTCAGTCCATGAGAT
ATTAGTTTTATAACTAACACAAACTTTTTTAAGTCACTCAAACACTGACGTGAACCTTAT
ACATATAAACCTTTTTAAGTTTCTTTTTCTTTTGCAGGTTACCAATCACTCTCCATGGAA
TACTCATTTGAGCAAGATGTTGGCATGAAATTGCCGTGGTGAAGGACGAGCTGACTCTAT
ATCGATGGTGCCTCGACCTCGATCCTTCACCTATTTATTGAAAGCATAGTTTGAATCTCA
TTTTCAACTATGTATAAATTAATATAAACAATGCAGGTCTAGAAAATAAGCTCTTTTTTT
TTCATTACCATTGA
   
Sequence Name 02-K034547-022-185-E01C-NB57
GenBank Accession LN486930 [GenBank]
Sequencing primer gene specific primer, NB57: 5'-TAGGCAGTCAATGGTAATGAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K034547-022-185-E01C-NB57
CTGCATTGTTTATTTAATTTATACATAGTTGAAAATGAGATTCAAACTATGCTTTCAATA
AATAGGTGAAGGATCGAGGTCGAGGCACCATCGATATAGAGTCAGCTCGTCCTTCACCAC
GGCAATTTCATGCCAACATCTTGCTCAAATGAGTATTCCATGGAGAGTGATTGGTAACCT
GCAAAAGAAAAAGAAACTTAAAAAGGTTTATATGTATAAGGTTCACGTCAGTGTTTGAGT
GACTTAAAAAAGTTTGTGTTAGTTATAAAACTAATATCTCATGGACTGACGTAAAGTTAA
TTAACTCTACGTAATTGTTGTTAATATCCTTCCCATTTTTCAATCTTACCTCATTTATAC
AAGTGTTGTTAATTAAGTGACCGGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCACTCCCNAATGG

Last Updated on 06.12.2018 16:11