Sequences from confirmation amplicon


Confirmation sequences for line 089H10 and hit At3g26060 are shown.
Line ID 089H10
Locus At3g26060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9525228
Primers used for PCR PL01 - 8474, show primer details
   
Sequence Name 07-K023833-022-089-H10C-8409
GenBank Accession LN489085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K023833-022-089-H10C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATATTGGGTTTTAAGTCTGAATTTAAATGTTA
TAAGAACAGATTTATGAATGTGTTAGTGCAGAATCAAAGACAAAATCAAAGAAACTTGTA
ATCAGTGTTTGTTTTAGAGACCATAAACTTTCACATACCTGAAAATAAGCAAATGCTAGA
ATGTTCTTGAATATGTTCAATCCTTTATTCATATGATTTAAGCTGACACGGCATATGAAC
TCTCTTTAACCTTCTGTGAATTGGTCTCTTTGTGATGTTTGGTGGGCAGCAGGTTAACAA
GGGTCAGGCTGCACCAGACTTCACACTAAAGGATCAAAACGGAAAGCCGGTGAGCCTCAA
AAAGTATAAAGGGAAGCCTGACCCTTGTTAACCTGCTGCCCACCAAACATCACAAAGAGA
CCAATTCACAGAAGGTTAAAGAGAGTTCATATGCCGTGTCAGCTTAAATCATATGAATAA
AGGATTGAACATATTCAAGAACATTCTAGCATTTGCTTATTTTCAGGTATGTGAAAGTTT
ATGGTCTCTAAAACAAACACTGATTACAAGTTTCTTTGATTTTGTCATTGATTCTGCACT
AACACATTCATAAATCTGTTCTTATAACATTTAAAT
   
Sequence Name 11-K023833-022-089-H10C-PL01
GenBank Accession LN490932 [GenBank]
Sequencing primer gene specific primer, PL01: 5'-CAGGCTTCCCTTTATACTTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K023833-022-089-H10C-PL01
GCTCACCGGCTTTTCCGTTTTGATCCTTTAGTGTGAAGTCTGGTGCAGCCTGACCCTTGT
TAACCTGCTGCCCACCAAACATCACAAAGAGACCAATTCACAGAAGGTTAAAGAGAGTTC
ATATGCCGTGTCAGCTTAAATCATATGAATAAAGGATTGAACATATTCAAGAACATTCTA
GCATTTGCTTATTTTCAGGTATGTGAAAGTTTATGGTCTCTAAAACAAACACTGATTACA
AGTTTCTTTGATTTTGTCTTTGATTCTGCACTAACACATTCATAAATCTGTTCTTATAAC
ATTTAAATTCAGACTTAAAACCCAATATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11