Sequences from confirmation amplicon


Confirmation sequences for line 257A06 and hit At3g29320 are shown.
Line ID 257A06
Locus At3g29320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11253551
Primers used for PCR SD20 - 8474, show primer details
   
Sequence Name 01-K103500-0022-257-A06C-SD20
GenBank Accession LN486540 [GenBank]
Sequencing primer gene specific primer, SD20: 5'-GTAACTGATTCGTATTCCTTGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103500-0022-257-A06C-SD20
AAGTGTTTATTAGCTCGATGAATCCGTTTGCGCCAGATGCTGCTTCGGTAGCTTCGAGTA
TCAAGTACCACGCGGAGTTTACGCCATTGTTTTCACCGGAGAAGTTTGAGTTGCCAAAGG
CGTTCTTTGCGACTGCGCAAAGTGTTAGAGATGCTTTGATCATGAATTGGAATGCAACTT
ATGAGTATTACAACAGAGTGAATGTGAAACAAGCGTATTATTTGTCAATGGAGTTTTTGC
AGGTTTTGGTTTTTACTCATTTCTTTGAGTGATTTTGTTCTTGGTTGTTATCTAACTATT
TTACATTGTAGGGTAGAGCAATGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGAAGTCCGCAGCGTTATT
   
Sequence Name 17-K103501-0022-257-A06C-8409
GenBank Accession LN493817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103501-0022-257-A06C-8409
GCCATTTACTCATTGCTCTACCCTACAATGTAAAATAGTTAGATAACAACCAAGAACAAA
ATCACTCAAAGAAATGAGTAAAAACCAAAACCTGCAAAAACTCCATTGACAAATAATACG
CTTGTTTCACATTCACTCTGTTGTAATACTCATAAGTTGCATTCCAATTCATGATCAAAG
CATCTCTAACACTTTGCGCAGTCGCAAAGAACGCCTTTGGCAACTCAAACTTCTCCGGTG
AAAACAATGGCGTAAACTCCGCGTGGTACTTGATACTCGAAGCTACCGAAGCAGCATCTG
GCGCAAACGGATTCATCGAGCTAATAAACACTTCTGAAAATCAATCAACACAAAAACAAA
ATCCCAAGGAATACGAATCAGTTACAGGGGGGGGGGGGG

Last Updated on 06.12.2018 16:11