Sequences from confirmation amplicon


Confirmation sequences for line 573G07 and hit At3g43160 are shown.
Line ID 573G07
Locus At3g43160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15168116
Primers used for PCR 27db - 8474, show primer details
   
Sequence Name 83-K116920-0022-573-G07C-27db
GenBank Accession LN514351 [GenBank]
Sequencing primer gene specific primer, 27db: 5'-CAGAAATTTCAATGTTTGTTGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K116920-0022-573-G07C-27db
TGAGGGAGTCTGGGCAGTCCATGGGAACCCCCTTACTCGGTTCGGACTTGGGTAGCGATC
GAGGGATACTATCGGATACCGACACGAGGAATGACCGACCAACCGTCCGACCAACCGACC
GTCCGACTGACTGTCCGACCGACTATCGACTTTTCCGACAATCGGCTTTTTTGCCTTTTT
TGAGTTTTACAGCAGTTTTCAGACAAAAATTGATGAATCGTCGAGGAGAATGGGCTTGCC
TAGCGTGGGCTACCATTAATTCTTCGAGGCGTTAAGGTGGCGGTGGTATAAAAGTGTCGG
AGTTTTTTCTGCAATTCTCAGACAAAAATTGTTGAGTGGTCGAGAAGAATGGGCATGTCA
TGTGTCGGCTGACATGGATTCTTCGAGGCCTAGGGTTGGCGGTATATAACTTTTGCGCAT
GATATTACCGAGATGTCCCCACGGGCATCGTTTCACCTCGTCGCCGAAGAGAATGGGCGT
GTCATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCG
   
Sequence Name 39-K116919-0022-573-G07C-8409
GenBank Accession LN503490 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116919-0022-573-G07C-8409
ATGACACGCCCATTCTCTTCGGCGACGAGGTGAAACGATGCCCGTGGGGACATCTCGGTA
ATATCATGCGCAAAAGTTATATACCGCCAACCCTAGGCCTCGAAGAATCCATGTCAGCCG
ACACATGACATGCCCATTCTTCTCGACCACTCAACAATTTTTGTCTGAGAATTGCAGAAA
AAACTCCGACACTTTTATACCACCGCCACCTTAACGCCTCGAAGAATTAATGGTAGCCCA
CGCTAGGCAAGCCCATTCTCCTCGACGATTCATCAATTTTTGTCTGAAAACTGCTGTAAA
ACTCAAAAAAGGCAAAAAAGCCGATTGTCGGAAAAGTCGATAGTCGGTCGGACAGTCAGT
CGGACGGTCGGTTGGTCGGACGGTTGGTCGGTCATTCCTCGTGTCGGTATCCGATAGTAT
CCCTCGATCGCTACCCAAGTCCGAACCGAGTAAGGGGGTTCCCATGGACTGCCCAGACTC
CCTCAACACCCACCCCCTATATAGCTTTATATCCTTTTTCCGACTTGGCACCAACAAAC

Last Updated on 06.12.2018 16:11