Sequences from confirmation amplicon


Confirmation sequences for line 774E06 and hit At3g43330 are shown.
Line ID 774E06
Locus At3g43330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15281724
Primers used for PCR NS34 - 8474, show primer details
   
Sequence Name 58-K102504-0022-774-E06C-8409
GenBank Accession LN509668 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K102504-0022-774-E06C-8409
ATTTACAATTGAATATATCCTGTAAAAGAAGCTGGAATTTTAACTTCTTTACCTTGGTTT
TCCAAATCATTGTTTCCAGAGTTCGATGCATTCTGAATTGGTTCTCTGGTAGAACTTCTG
AATTTTGGTTGATTCTTCTTAATATACCTCAGCCTGTTAGACTCAATCATAGTGTAAGCA
TCAACTATGAACTGCTGCAGCAAACGCTTAGAACGAAATAAAATGTGTTTCTCCGCCTTC
CTTTCTTGTAGACGATATGCAAACCATTGTCTCATGCTTACGTCTTTCTGTTCTTTTTTT
CCATCGCCATGACCATTTGTTCTAGCTTTCTTTATGCCAAGTCTATAACCATCTTCCCCG
CGTGCGAATAATAGAGGATATTGCAAAGCTAGGTAGGAAACATGTAGTTAACTGATTCTT
TTTAACTTCCCCGAAGTAGTTTCCTGTAACACAATATCAAGTTTATCCATGTCTTCATGG
AAATCTCCTGGAATTAATGCAGCAACTTCAGATGCAGTAGGCAGATTGTATATCCTTGGG
TCAGTTTTTCGAGCAGCAATAATTCTCATGTGAAAACTTTCTTGCGCATCTGTATTAAAT
CTGTCTCTTGCATATCTAATGCCCTTCAC
   
Sequence Name 58-K102505-0022-774-E06C-NS34
GenBank Accession LN509669 [GenBank]
Sequencing primer gene specific primer, NS34: 5'-TGTGAAGGCATTTAGATATGCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K102505-0022-774-E06C-NS34
AGAAGTTTTCACATGAGAATATTGCTGCTCGAAAAACTGACCCAAGGATATACAATCTGC
CTACTGCATCTGAAGTTGCTGCATTAATTCCAGGAGATTTCCATGAAGACATGGATAAAC
TTGATATTGTGTTACAGGAAACTACTTCGGGGAAGTTAAAAAGAATCAGTTAACTACATG
TTTCCTACCTAGCTTTGCAATATCCTCTATTATTCGCACGCGGGGAAGATGGTTATAGAC
TTGGCATAAAGAAAGCTAGAACAAATGGTCATGGCGATGGAAAAAAAGAACAGAAAGACG
TAAGCATGAGACAATGGTTTGCATATCGTCTACAAGAAAGGAAGGCGGAGAAACACATTT
TATTTCGTTCTAAGCGTTTGCTGCAGCAGTTCATAGTTGATGCTTACACTATGATTGAGT
CTAACAGGCTGAGGTATATTAAGAAGAATCAACCAAAATTCAGAAGTTCTACCAGAGAAC
CAATTCAGAATGCATCGAACTCTGGAAACAATGATTTGGAAAACCAAGGTAAAGAAGTTA
AAATTCCAGCTTCTTTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATCACCCCCAAAAGGGG

Last Updated on 06.12.2018 16:11