Sequences from confirmation amplicon


Confirmation sequences for line 314D04 and hit At3g44460 are shown.
Line ID 314D04
Locus At3g44460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16080365
Primers used for PCR NT10 - 8474, show primer details
   
Sequence Name 60-K036524-022-314-D04C-NT10
GenBank Accession LN510188 [GenBank]
Sequencing primer gene specific primer, NT10: 5'-TATACTGTGGAACTGGAGTTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K036524-022-314-D04C-NT10
GAAGCTGAAGGAATTGTGGTAATTTCTTGATAAAACATTATTTATAACGACTAATATAAG
CTATTAAGCTATGATTCATATTTGGAAAACAATATCCTAGTAACCATTTTCAATTTAATA
CTTTTTGCTAGGAGGAAAATGAGAAGAAAAGAAGACAAGAGGTAATACTCCAATTTCCAT
ATGTACTAAAACGGTTACTTAACGAATATGAATTATCAAAATACATCATTTTGGATTTAT
GTGTGATATTGTTGGATTCAAAGTTGACAATATCGTTATCTAACAAATATAGCTTAAATG
ATAATAAGTTAATAACTAAGCTGTGATGGGTTAGTGGTTTTTATTTGAAGTATAAATCAA
GCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 36-K036524-022-314-D04C-8409
GenBank Accession LN502133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K036524-022-314-D04C-8409
CATTTAAATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGATTTA
TACTTCAAATAAAAACCACTAACCCATCACAGCTTAGTTATTAACTTATTATCATTTAAG
CTATATTTGTTAGATAACGATATTGTCAACTTTGAATCCAACAATATCACACATAAATCC
AAAATGATGTATTTTGATAATTCATATTCGTTAAGTAACCGTTTTAGTACATATGGAAAT
TGGAGTATTACCTCTTGTCTTCTTTTCTTCTCATTTTCCTCCTAGCAAAAAGTATTAAAT
TGAAAATGGTTACTAGGATATTGTTTTCCAAATATGAATCATAGCTTAATAGCTTATATT
AGTCGTTATAAATAATGTTTTATCAAGAAATTACCACAATTTCCTTCAGCTTCGTGTTTT
CTTCCGTGAGGTTGTTCAATTCCAACTCCAGTCCAACAGTAT

Last Updated on 06.12.2018 16:11