Sequences from confirmation amplicon


Confirmation sequences for line 152C12 and hit At3g46010 are shown.
Line ID 152C12
Locus At3g46010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16909306
Primers used for PCR VL29 - 8474, show primer details
   
Sequence Name 25-K020888-022-152-C12C-8409
GenBank Accession LN497249 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K020888-022-152-C12C-8409
TAGATTTCCCGGACATGAAGCCATTTCTTTGGAAACAAACATAAGAATGAATGGTCAAAA
CGACCAATCCAATCATAATCAGTGGACCAAATAAAAAAACTGAAGATCAATCTTGTCCTA
TAGACAGTTGTTTCATACAAATGTGAGAGAAACTTTTAATCATCCATTTCATCAATGATA
TACCTAAAAGGGTTCATCTTCAATAGACGATTTGGGGTCCTTACACACCATTTTAAAACC
AAAGACAACATCGAACACAAGACCGAAACACCGATAGAATGAAGTTGTTTATAGGTTCAT
AACTTCACAACACAATGTAAAAACATAATCAAGTACCAGTACCATCACTCAAAAATAACA
GAATAAGAGAATAGATCTTTCAAGGGGATGATGATAATAAACTTTGGTTTTTACTTTTTA
GTTGGCACGGCTCCTGAAAACATCGAGATCCATCTCTGTTGGATCAGTTGCCTGTAGCTC
TACTTGAATCCCATCTAGTTCCCTC
   
Sequence Name 26-K020888-022-152-C12C-VL29
GenBank Accession LN497725 [GenBank]
Sequencing primer gene specific primer, VL29: 5'-ACAGGTTCAAGAGGGAACTAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K020888-022-152-C12C-VL29
GATTCAGTAGAGCTACGGCAACTGATCCAACAGAGATGGATCTCGATGTTTTCAGGAGCC
GTGCCAACTAAAAAGTAAAAACCAAAGTTTATTATCATCATCCCCTTGAAAGATCTATTC
TCTTATTCTGTTATTTTTGAGTGATGGTACTGGTACTTGATTATGTTTTTACATTGTGTT
GTGAAGTTATGAACCTATAAACAACTTCATTCTATCGGTGTTTCGGTCTTGTGTTCGATG
TTGTCTTTGGTTTTAAAATGGTGTGTAAGGACCCCAAATCGTCTATTGAAGATGAACCCT
TTTAGGTATATCATTGATGAAATGGATGATTAAAAGTTTCTCTCACATTTGTATGAAACA
ACTGTCTATAGGACAAGATTGATCTTCAGTTTTTTTATTTGGTCCACTGATTATGATTGG
ATTGGTCGTTTTGACCATTCATTCTTATGTTTGTTTCCAAAGAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11