Sequences from confirmation amplicon


Confirmation sequences for line 435B04 and hit At3g46350 are shown.
Line ID 435B04
Locus At3g46350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17037285
Primers used for PCR EQ69 - 8474, show primer details
   
Sequence Name 39-K103204-0022-435-B04C-8409
GenBank Accession LN503317 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103204-0022-435-B04C-8409
CAGCCATTTTTCCGGAGTATATATTTCAGCCGGAATATTCAAGATGTCCCGGCATTGCTG
ATCATTGTTTATGTTATATGCCTAATGGCTAAAATTGCCATTAACCTTTTGCCATATCTT
AACAGGATTCATAAGTTTGGATTGTGGGTTAGCTCCGACTGAGCCGTCCCCTTATACCGA
GCCGGTAACTACGTTACAGTACTCATCGGATTCAAACTTCATCCAAAGTGGGAAACTTGG
TAGAATTGATACAAGTCTACAGACATTTTTCCTAAAGCAACAAACAACGTTAAGATACTT
TCCAGACGGAATACGCAATTGTTACAATCTTACGGTCAAGCAGGGAACCAACTATTTGAT
CAGAGCTAGGTTCACATATGGGAATTATGATGGTCGTAATATGTCTCCTACCTTTGATCT
ATACTTGGGCCCCAATTTGTGGAAAAGAATAGACATGACAAAGTTACAAAACAAAGTTAG
TACATTGGAGGAGATTACATACATTCCATTATCAAACTCTTTGGATGTGTGTCTTGTTAA
GACAAACACGACCATTCCNTTTAT
   
Sequence Name 40-K103204-0022-435-B04C-EQ69
GenBank Accession LN503672 [GenBank]
Sequencing primer gene specific primer, EQ69: 5'-TCTAAGGCCGATATAAATGGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K103204-0022-435-B04C-EQ69
ATCCAAGAGTTTGATATGGAATGTATGTAATCTCCTCCATGTACTAACTTTGTTTTGTAA
CTTTGTCATGTCTATTCTTTTCCACAAATTGGGGCCCAAGTATAGATCAAAGGTAGGAGA
CATATTACGACCATCATAATTCCCATATGTGAACCTAGCTCTGATCAAATAGTTGGTTCC
CTGCTTGACCGTAAGATTGTAACAATTGCGTATTCCGTCTGGAAAGTATCTTAACGTTGT
TTGTTGCTTTAGGAAAAATGTCTGTAGACTTGTATCAATTCTACCAAGTTTCCCACTTTG
GATGAAGTTTGAATCCGATGAGTACTGTAACGTAGTTACCGGCTCGGTATAAGGGGACGG
CTCAGTCGGAGCTAACCCACAATCCAAACTTATGAATCCTGTTAAGATATGGCAAAAGGT
TAATGGCAATTTTAGCCATTAGGCATATAACATAAACCATGATCAGCAATGCCGGGACAT
CTNGAATATTCCGGCTGAAATATATACTCCGGAAAAATGGCTTGCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCACAAATGTAGA

Last Updated on 06.12.2018 16:11