Sequences from confirmation amplicon


Confirmation sequences for line 447D01 and hit At3g46560 are shown.
Line ID 447D01
Locus At3g46560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17138667
Primers used for PCR AS53 - 8474, show primer details
   
Sequence Name 07-K105974-0022-447-D01C-AS53
GenBank Accession LN489275 [GenBank]
Sequencing primer gene specific primer, AS53: 5'-TGACAACAACACACAAAATGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105974-0022-447-D01C-AS53
GACATTTCCTAACTCTAATAAAACTTTTGTTTATCAATGAACCATAAGGACCCAAAGATC
TATAATTAGCCAGAAATAAGTACACACTAACCTTGAATCAATATTCTAAAGCAAATCTAA
TTAGCTTTGAACTATAAGAACAAAGATTTCAAGGAGCTAAAAGTCAATTTTTTTGGGAGA
ATCCTAAATGAAAAACAGAGAAACCGATCTAAGTGACGAACCTATCACGGAGCTGAAGCT
GATCGATCATGGAGGCCATTTTGGCTTTGTCTTCTTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 17-K105975-0022-447-D01C-8409
GenBank Accession LN493856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K105975-0022-447-D01C-8409
TTCGGACATGAGCCATTTACAATTGAATATATCCTGAAGAAGACAAAGCCAAAATGGCCT
CCATGATCGATCAGCTTCAGCTCCGTGATAGGTTCGTCACTTAGATCGGTTTCTCTGTTT
TTCATTTAGGATTCTCCCAAAAAAATTGACTTTTAGCTCCTTGAAATCTTTGTTCTTATA
GTTCAAAGCTAATTAGATTTGCTTTAGAATATTGATTCAAGGTTAGTGTGTACTTATTTC
TGGCTAATTATAGATCTTTGGGTCCTTATGGTTCATTGATAAACAAAAGTTTGGATTAGA
GTTAGTGAAATTGTTCTTTAAGATGTAACTGTGTTGTCTCATTTTGTGGTNTGTTGTCA

Last Updated on 06.12.2018 16:11