Sequences from confirmation amplicon


Confirmation sequences for line 687H08 and hit At3g47180 are shown.
Line ID 687H08
Locus At3g47180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17372948
Primers used for PCR CQ87 - 8474, show primer details
   
Sequence Name 41-K107133-0022-687-H08C-CQ87
GenBank Accession LN504096 [GenBank]
Sequencing primer gene specific primer, CQ87: 5'-GATTCATATATCACCGTGCCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K107133-0022-687-H08C-CQ87
TAACTTAAAAAAAAAATATGTAATGTTTATAACCAAAATGTAAGTTTATTTTCTAAATTC
TTCAATTTTCTTTTCTGGTTAATTTGTTATAAGAATGAGTATATTTACTAAGTCTCAATA
GCTCAACTAGTAAAATACCCCATGCAAAACCTAGAGGTCGTAGGTTTAATCCCCCAAAAA
AGTTAATAAACTAATTAATGATAATTAAAATATTTATACCATTACATTTCAACTGACAGA
TGGTTCAGAACAACAAATGGGACAAATCTTCTTTGTCTCAAGCCATTTGGTTATACACTC
AGAGTGGTATGGATGGTCACAAGGTCGGAGAACGACCAAAGATTCTCTTTCTTCAATTGG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 33-K107132-0022-687-H08C-8409
GenBank Accession LN501020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K107132-0022-687-H08C-8409
AATTGAAGAAGAGAATCTTTGGTCGTTCTCCGACCTTGTGACCATCCATACCACTCTGAG
TGTATAACCAAATGGCTTGAGACAAAGAAGATTTGTCCCATTTGTTGTTCTGAACCATCT
GTCAGTTGAAATGTAATGGTATAAATATTTTAATTATCATTAATTAGTTTATTAACTTTT
TTGGGGGATTAAACCTACGACCTCTAGGTTTTGCATGGGGTATTTTACTAGTTGAGCTAT
TGAGACTTAGTAAATATACTCATTCTTATAACAAATTAACCAGAAAAGAAAATTGAAGAA
TTTAGAAAATAAACTTACATTTTGGTTATAAACATTACATATTTTTATTTATTAAGTTTA
AGGAATCAAAGATATATACGTAAAACAAGAGGCACGGTGAATTATGAAT

Last Updated on 06.12.2018 16:11