Sequences from confirmation amplicon


Confirmation sequences for line 665C07 and hit At3g47190 are shown.
Line ID 665C07
Locus At3g47190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17376107
Primers used for PCR CQ66 - 8474, show primer details
   
Sequence Name 06-K107108-0022-665-C07C-8409
GenBank Accession LN488836 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K107108-0022-665-C07C-8409
CATTTACATTGAATATATCCATGTAGATTTCCCGGACATAAAGCCATTTTCATGTCGTTT
TCAATTTGCATTACCCCTTATTCACAAGAGAGATAGAGAGATATCAGAAACAACGGGTGC
ACTTCAAAGGAGAACATGAGCAACTCGGAGTCTTATCCACCAGAGTTTCGACCTCTAATG
AGCGAAAAATCCACTGAAACGGGTCTAGACCGGAGCAAAGATATCGATATCCCGGTTATC
GATATGGAGCATTTGGACATGGAGAAACTGAGAGAAGCATGCAAAGACTGGGGGATCTTC
CATTTGGAGAATACTGGGATACCCTTAACGTTTATGTCACAGGTGAAAGAGA
   
Sequence Name 22-K107113-0022-665-C07C-CQ66
GenBank Accession LN496137 [GenBank]
Sequencing primer gene specific primer, CQ66: 5'-TCTCTTTCACCTGTGACATAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K107113-0022-665-C07C-CQ66
CAATGGAGATCCCCCAGTCTTTGCATGCTTCTCTCAGTTTCTCCATGTCCAAATGCTCCA
TATCGATAACCGGGATATCGATATCTTTGCTCCGGTCTAGACCCGTTTCAGTGGATTTTT
CGCTCATTAGAGGTCGAAACTCTGGTGGATAAGACTCCGAGTTGCTCATGTTCTCCTTTG
AAGTGCACCCGTTGTTTCTGATATCTCTCTATCTCTCTTGTGAATAAGGGGTAATGCAAA
TTGAAAACGACATGAAAATGGCTTTATGTCCGGGAAATCTACATGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
ATTATT

Last Updated on 06.12.2018 16:11