Sequences from confirmation amplicon


Confirmation sequences for line 232H08 and hit At3g48090 are shown.
Line ID 232H08
Locus At3g48090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17757543
Primers used for PCR 22lf - 8474, show primer details
   
Sequence Name 70-K113920-0022-232-H08C-8409
GenBank Accession LN512428 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113920-0022-232-H08C-8409
TTTCTCTGANAAGATGGTTGGAAGCGAAGATTACGACTGCTCCTGCTTCTTCCTTGTGAT
AGCGCTCGGTTAGGTAAGCTTGCTTCGAGGCTGACCATGATCTGGTGATTAGATCACCAT
TGATTCCGGTAAGAGCTTCAAACGCCATTGATCTATATCTATTCTCTTTTCTTTAGTGGA
CTTTCTTCTCTCTTTTTTCTTTTGAATCGTCTTGCTTCTTTAATCCGCTCCTACTCTGTT
AATTTATGGGAAGATGTAGACAGGACTAATTAGTTACCCAAAATACCCCATCATGAGACC
ATTTCAATGCAAAAATGGGTTTTTTTTTTTGGAGTTAACTCTTTGGCTATGGGAAACTCA
GGAAAAAAAAAAAGCTGGGAATCTAACCTTTCCACGTGTCGGTTTAAC
   
Sequence Name 60-K113919-0022-232-H08C-22lf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 22lf: 5'-AATGTAAAACCGACACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113919-0022-232-H08C-22lf
CTCCATAGCCAAGAGTCAACTCCAAAAAAAAAACCCTTTTTGGCATCGAAAGGGTCCCAG
GAGGGGGTATTTGGGGAAACTAATTAGCCCGGTCTACATCTTCCCATAATTTAACAAAGT
AGGAGCGGATTAAAAAACCAGGACAATTCAAAAGAAAAAAGAGAGAAAAAGGTCCCCTAA
GGAAAAGAGAATAGATTTGGATCATGGGCTTTGGAAGCTCTAACCGGAATCAAGGGGGAT
CTAATCCCCAAATCAGGGTCAGCCTCAAAGCAGGCTTACCTAACCAAGCGCTTTCACAGG
GAAAAACCGGGACCAGTCGTAATCTTCGTTTTCCAACCATCTTTCTAAAAAAAAGGGCTT
CTTGTCCGGGAAATCTACTGGGATCACCATTGAGTATGAGGGTCAATAGGGAAAAAAAAA
AAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCCC

Last Updated on 06.12.2018 16:11