Sequences from confirmation amplicon


Confirmation sequences for line 432E01 and hit At3g48400 are shown.
Line ID 432E01
Locus At3g48400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17925559
Primers used for PCR NU13 - 8474, show primer details
   
Sequence Name 29-K119947-0022-432-E01C-NU13
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NU13: 5'-GTGTTCAACAACAAACCATTTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K119947-0022-432-E01C-NU13
ATTGGTTTGAGTGTGATGCTTGTAGAAAATCTCCAATGGTTTTAGATACGTTTCTGGTGA
TTGGATTCTAGATGTGCATTGCGGTTCGATGTCAGAACCATTGCTCCATGATGGTCATTC
ACATCCGTTATATTACTACAGAAGGCAAGGCAAGTCATGTCGTTCGTGTCACAACAAAGT
GGTGAAACCTAGAGTACTATGTTGTGATACTTGTGACTTTGATTTGGATTTCTACTGTGC
TAATTTACCTAAAACGGTAAAACATTGCTACGACGAGCATCCTCTTTCTCTATGCCACGG
CGAAGATGATGTGATTAAGGATAACAAATATTGGTGTGACATATGCGAAACAGAAACCGA
TCCAAAGAAATGGTTCTACACTTGTTATGACTGCGGGGTAACTTTACATGTCAAGTGTGT
ACTGGGAGACTTCTCGCGTCTCATGCCAGGGCGGAACATTAATGTCGATGGACGTGAGTA
TGAAGTTGTTCCTAACAGCAAGAGTTCTCGACCATTCTGCGACCAATGCCTTGATTTCCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATAAGT
ATGATGGTCAATAT
   
Sequence Name 09-K119946-0022-432-E01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K119946-0022-432-E01C-8409
CATTACATTGAATATATCCTGGAAATCAGGCATTGGTCGCAGAATGGTCGAGAACTCTTG
CTGTTAGGAACAACTTCATACTCACGTCCATCGACATTAATGTTCCGCCCTGGCATGAGA
CGCGAGAAGTCTCCCAGTACACACTTGACATGTAAAGTTACCCCGCAGTCATAACAAGTG
TAGAACCATTTCTTTGGATCGGTTTCTGTTTCGCATATGTCACACCAATATTTGTTATCC
TTAATCACATCATCTTCGCCGTGGCATAGAGAAAGAGGATGCTCGTCGTAGCAATGTTTT
ACCGTTTTAGGTAAATTAGCACAGTAGAAATCCAAATCAAAGTCACAAGTATCACAACAT
AGTACTCTAGGTTTCACCACTTTGTTGTGACACGAACGACATGACTTGCCTTGCCTTCTG
TAGTAATATAACGGATGTGAATGACCATCATGGAGCAATGGTTCTGACATCGAACCGCAA
TGCACATCTAGAATCCAATCACCAGAAACGTATCTAAAACCATTGGAGATTGTTCTACAA
GCATCACACTCAAACCAATTCCTAGAAGAATCTTTACCTCCTGTGTGTAACGTAAATGGT
TTGATGTTGAACACAGNCNAGGTGGTTAATATTCTTCTAGGAATTGGTTTGAGTGTGATG
CTTGTAGAAAAATCTCCAATGGTTTTAGATACGTTTCTGGTGATTGGATTCTAGATGTGC
ATT
Primers used for PCR 40yf - 3144, show primer details
   
Sequence Name 30-K119947-0022-432-E01C-40yf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 40yf: 5'-CTGATGGGTTAGGACTAGGACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K119947-0022-432-E01C-40yf
ATGGGCATGCCTTAACTACATTCCTCACAGGTACTGCATTTGAATCTGTCTTATTGGTTT
TGGAAATGTTTTCGATGTATCGAGAAAGCTATTAACTTTGATCTTGAAACAATAGCTAAG
ATGTGTTGTGAAATGTGTTTTTTTGGTTATGGACTTTGTTTACAGGTTAGCAGGAGCTGC
CCTTGTAGCTCCAGCGATTAACTATTGGTGGAGAAACTTACCGGGAGATTTAACAAGAGA
AGCTTTCTCTCTTATGCATCCTGCAGATCAATGGTCACTTCGAGTAGCTCATTATGCTCC
TTGGCTTACATATTGGTGGAACACTCAGAAATGGTTCCCAATCTCCAATGTGATTGCCGG
TAATCCCATTATTTTCTCACGTCAGGACATGGAGATCTTGTCGAAGCTCGGATTCGTCAA
TCCAAATCGGGTACAAGCACATCTATAGACGTTCAGTTTCCATATCATAAGTTTCATACC
TCTACAATGAGAAGATGATTTGGAATCATATGATGTGTATATTCCTTTTTTTTTGTTCCA
GGCATACATAAGACAACAAGGTGAATATGTAAGCTTACACCGAGATTTGAATGTCGCATT
TTCAAGCTGGGAGTTTGATCCGTTAGACCTTCAAGATCCGTTCCCGAACAACAATGGCTC
AGTTCACGTATGGAATGGCGATGAGGATAAGTTTGTGCCAGTAAAGCTTCAACGGTATGT
CGCGTCAAAGCTGCCATGGATTCGTTACCATGAAATATCTGGATCAGGACATTTTGTACC
ATTTGTGGAAGGTATGACTGATAAGATCATCAAGTC
   
Sequence Name 10-K119946-0022-432-E01C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119946-0022-432-E01C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGATA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGAA
TAATTGCAATGTTGTTAATTGTAATCTATGCGTTGATGTCTACATTTGCTCCCGTAAATG
CCGCCAGCCCTTTCATTTGAGACATTAGTACACTTGTATTAACGACTAAAGTTGCTTTGA
TTTGATTGTTGATCATAACTAGTTTAGCATTGCAAGCAATTAATTATTCTTAAAAACCAC
ATTGACTTAAACAGAGGCTTCTCTACTCTCACTTACATCTTCTTCCCCAACCAAAAGTGA
CTTGATGATCTTATCAGTCATACCTTCCACAAATGGTACAAAATGTCCTGATCCAGATAT
TTCATGGTAACGAATCCATGGCAGCTTTGACGCGACATACCGTTGAAGCTTTACTGGCAC
AAACTTATCCTCATCGCCATTCCATACGTGAACTGAGCCATTGTTGTTCGGGAACGGATC
TTGAAGGTCTAACGGATCAAACTCCCAGCTTGAAAATGCGACATTCAAATCTCGGTGTAA
GCTTACATATTCACCTTGTTGTCTTATGTATGCCTGGAACAAAAAAAAAGGAATATACAC
ATCATATGATTCCAAATCATCTTCTCATTGTAGAGGTATGAAACTTATGATATGGAAACT
GAACGTCTATAAATGTGCTTGTACCCGATTTGGATTGAC

Last Updated on 06.12.2018 16:11